Effect of Cadmium Chloride on Metallothionein Levels in Carp

KOVAROVA, Jana, Rene KIZEK, Vojtech ADAM, Danka HARUŠTIAKOVÁ, Olga CELECHOVSKA and Zdenka SVOBODOVA. Effect of Cadmium Chloride on Metallothionein Levels in Carp. Sensors. Basel, Switzerland: Molecular Diversity Preservation Interna, 2009, vol. 9, No 6, p. 4789-4803. ISSN 1424-8220.

Basic information

• Original name: Effect of Cadmium Chloride on Metallothionein Levels in Carp
• Name in Czech: Effect of Cadmium Chloride on Metallothionein Levels in Carp
• Authors: KOVAROVA, Jana (203 Czech Republic), Rene KIZEK (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Danka HARUŠTIAKOVÁ (703 Slovakia, guarantor), Olga CELECHOVSKA (203 Czech Republic) and Zdenka SVOBODOVA (203 Czech Republic).
• Edition: Sensors, Basel, Switzerland, Molecular Diversity Preservation Interna, 2009, 1424-8220.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10511 Environmental sciences
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.821
• RIV identification code: RIV/00216224:14310/09:00035776
• Organization unit: Faculty of Science
• UT WoS: 000267339700044
• Keywords (in Czech): heavy metals; bioaccumulation; Cyprinus carpio
• Keywords in English: heavy metals; bioaccumulation; Cyprinus carpio
• Tags: bioaccumulation, Cyprinus carpio, heavy metals
• Tags: International impact, Reviewed

Abstract

Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.

Abstract (in Czech)

Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)