Activation via the antigen receptor is impaired in T cells, but
not in B cells from patients with common variable
immunodeficiency

J 1996

Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency

FISCHER, MB., HM. WOLF, I. HAUBER, H. EGGENBAUER, Vojtěch THON et. al.

Basic information

Original name

Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency

Name in Czech

Aktivace cestou antigenního receptoru je porušena u T buněk, avšak ne u B buněk pacientů s běžnou variabilní imunodeficiencí

Authors

FISCHER, MB. (40 Austria), HM. WOLF (40 Austria), I. HAUBER (276 Germany), H. EGGENBAUER (40 Austria), Vojtěch THON (203 Czech Republic, guarantor), M. SASGARY (40 Austria) and MM. EIBL (40 Austria)

Edition

EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 0014-2980

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/96:00035801

Organization unit

Faculty of Medicine

UT WoS

A1996UD13800035

Keywords (in Czech)

TCR; CVID

Keywords in English

T cell activation; common variable immunodeficiency; T cell receptor; inositol phosphates

Abstract

ORIG CZ

V originále

The patients included in this study belong to a subset of common variable immunodeficiency (CVID) patients whose peripheral blood T cells have a T cell receptor (TCR)-mediated activation defect leading to impaired expression of the interleukin (IL)-2 gene upon stimulation with recall antigens (tetanus toxoid, Escherichia coli) or superantigens (staphy lococcal enterotoxins). In the present report we demonstrate that the patients' peripheral blood T cells failed to generate the second messenger inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) following stimulation with superantigen or mAb specific for the monomorphic region of the TCR beta-chain. Patients' T cell lines were also impaired in generating Ins(1,4,5)P, when stimulated with tetanus toxoid-pulsed autologous monocytes. Addition of a second or third co-stimulatory signal provided by recombinant IL-2, CD28 or both had no effect on the Ins(1,4)P-3 formation of the patients' antigen-driven T cell lines. The T cell activation defect, however, was not absolute, as Ins(1,4,5)P-3 formation in the patients T cells after phytohemagglutinin or aluminium fluoride stimulation was normal. The impairment in signal transduction via the T cell antigen receptor was limited to the patients' T cells, as no activation defect after ligation of surface immunoglobulin. the antigen receptor on B cells, could be detected.

In Czech

Aktivace cestou antigenního receptoru je porušena u T buněk, avšak ne u B buněk pacientů s běžnou variabilní imunodeficiencí.

Citovat

FISCHER, MB., HM. WOLF, I. HAUBER, H. EGGENBAUER, Vojtěch THON, M. SASGARY and MM. EIBL. Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency. EUROPEAN JOURNAL OF IMMUNOLOGY. 1996, vol. 26, No 1, p. 231-237. ISSN 0014-2980.

@article{837107,
   author = {Fischer, MB. and Wolf, HM. and Hauber, I. and Eggenbauer, H. and Thon, Vojtěch and Sasgary, M. and Eibl, MM.},
   article_number = {1},
   keywords = {T cell activation; common variable immunodeficiency; T cell receptor; inositol phosphates},
   language = {eng},
   issn = {0014-2980},
   journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
   title = {Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency},
   volume = {26},
   year = {1996}
}

TY  - JOUR
ID  - 837107
AU  - Fischer, MB. - Wolf, HM. - Hauber, I. - Eggenbauer, H. - Thon, Vojtěch - Sasgary, M. - Eibl, MM.
PY  - 1996
TI  - Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency
JF  - EUROPEAN JOURNAL OF IMMUNOLOGY
VL  - 26
IS  - 1
SP  - 231-237
EP  - 231-237
SN  - 00142980
KW  - T cell activation
KW  - common variable immunodeficiency
KW  - T cell receptor
KW  - inositol phosphates
N2  - The patients included in this study belong to a subset of common variable immunodeficiency (CVID) patients whose peripheral blood T cells have a T cell receptor (TCR)-mediated activation defect leading to impaired expression of the interleukin (IL)-2 gene upon stimulation with recall antigens (tetanus toxoid, Escherichia coli) or superantigens (staphy lococcal enterotoxins). In the present report we demonstrate that the patients' peripheral blood T cells failed to generate the second messenger inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) following stimulation with superantigen or mAb specific for the monomorphic region of the TCR beta-chain. Patients' T cell lines were also impaired in generating Ins(1,4,5)P, when stimulated with tetanus toxoid-pulsed autologous monocytes. Addition of a second or third co-stimulatory signal provided by recombinant IL-2, CD28 or both had no effect on the Ins(1,4)P-3 formation of the patients' antigen-driven T cell lines. The T cell activation defect, however, was not absolute, as Ins(1,4,5)P-3 formation in the patients T cells after phytohemagglutinin or aluminium fluoride stimulation was normal. The impairment in signal transduction via the T cell antigen receptor was limited to the patients' T cells, as no activation defect after ligation of surface immunoglobulin. the antigen receptor on B cells, could be detected.
ER  -

FISCHER, MB., HM. WOLF, I. HAUBER, H. EGGENBAUER, Vojtěch THON, M. SASGARY and MM. EIBL. Activation via the antigen receptor is impaired in T cells, but not in B cells from patients with common variable immunodeficiency. \textit{EUROPEAN JOURNAL OF IMMUNOLOGY}. 1996, vol.~26, No~1, p.~231-237. ISSN~0014-2980.

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