Interaction between Rtg2p and Mks1p in the regulation of the
RTG pathway of Saccharomyces cerevisiae.

J 2005

Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.

ŠPÍREK, Mário, JR FERREIRA JÚNIOR a Ronald BUTOW

Základní údaje

Originální název

Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.

Název česky

Interakce mezi Rtg2p a Mks1p v regulaci RTG dráhy Saccharomyces cerevisiae

Autoři

ŠPÍREK, Mário (703 Slovensko, garant), JR FERREIRA JÚNIOR (76 Brazílie) a Ronald BUTOW (840 Spojené státy)

Vydání

Gene, Elsevier, 2005, 0378-1119

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.694

Kód RIV

RIV/00216224:14310/05:00035909

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000231497200002

Klíčová slova česky

retrogradni regulace, RTG dráha, Rtg2, Mks1, kvasinka

Klíčová slova anglicky

Retrograde regulation; RTG pathway; Rtg2p; Mks1p; Yeast

Štítky

Mks1p, Retrograde regulation, RTG pathway, Rtg2p, Yeast

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 6. 2009 12:26, RNDr. Mário Špírek, PhD.

Anotace

ORIG CZ

V originále

Retrograde signaling mediates nuclear gene expression in response to changes in the functional state of mitochondria. In budding yeast, retrograde signaling, also termed the RTG pathway, relies on the heterodimeric, basic helix-loop-helix zipper transcription factors, Rtg1p and Rtg3p, for the activation of target gene expression. Activation of the RTG pathway leads to partial dephosphorylation of Rtg3p and its translocation, together with Rtg1p, from the cytoplasm to the nucleus. These processes depend on a positive regulatory factor, Rtg2p, a novel protein with a ATP binding domain similar to that of the Hsp70/actin/sugar kinase superfamily. Four negative regulatory factors, Lst8p, Mks1p, and two redundant 14-3-3 proteins, Bmh1/2p, function between Rtg2p and Rtg1/3p. Alternative interaction between Mks1p and Rtg2p or Bmh1/2p provides a means for regulation of the RTG pathway. When the RTG pathway is on, Mks1p is inactivated by its association with Rtg2p; and when the RTG pathway is off, Mks1p dissociates from Rtg2p and forms a complex with Bmh1/2p, which is the negative regulatory form of Mks1p. Here we show that Rtg2p and Mks1p can interact in the absence of other factors, and is thereby the minimal binary switch for regulation of the RTG pathway. Gel filtration experiments indicate that both Rtg2p and Mks1p exist in high molecular weight complexes. In response to changes in the activity of the RTG pathway, both Rtg2p and Mks1p shift to different sized high molecular weight complexes. Together, our data suggest that dynamic association between Mks1p and Rtg2p in high molecular weight complexes provides a means to regulate the RTG pathway.

Česky

Experimenty využívajíc gelovou filtraci naznačili, že Mks1 i Rtg2 existují ve vysoko molekulárních komplexech. Velkost techto komplexú reaguje na aktivitu RTG dráhy.

Citovat

ŠPÍREK, Mário, JR FERREIRA JÚNIOR a Ronald BUTOW. Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae. Gene. Elsevier, 2005, roč. 354, jul 18, s. 2-8, 8 s. ISSN 0378-1119.

@article{837972,
   author = {Špírek, Mário and Ferreira Júnior, JR and Butow, Ronald},
   article_number = {jul 18},
   keywords = {Retrograde regulation; RTG pathway; Rtg2p; Mks1p; Yeast},
   language = {eng},
   issn = {0378-1119},
   journal = {Gene},
   title = {Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-4GFCPV2-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=b0248dee1da31fecb053bc32145972b2},
   volume = {354},
   year = {2005}
}

TY  - JOUR
ID  - 837972
AU  - Špírek, Mário - Ferreira Júnior, JR - Butow, Ronald
PY  - 2005
TI  - Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.
JF  - Gene
VL  - 354
IS  - jul 18
SP  - 2-8
EP  - 2-8
PB  - Elsevier
SN  - 03781119
KW  - Retrograde regulation
KW  - RTG pathway
KW  - Rtg2p
KW  - Mks1p
KW  - Yeast
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-4GFCPV2-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=b0248dee1da31fecb053bc32145972b2
N2  - Retrograde signaling mediates nuclear gene expression in response to changes in the functional state of mitochondria. In budding yeast, retrograde signaling, also termed the RTG pathway, relies on the heterodimeric, basic helix-loop-helix zipper transcription factors, Rtg1p and Rtg3p, for the activation of target gene expression. Activation of the RTG pathway leads to partial dephosphorylation of Rtg3p and its translocation, together with Rtg1p, from the cytoplasm to the nucleus. These processes depend on a positive regulatory factor, Rtg2p, a novel protein with a ATP binding domain similar to that of the Hsp70/actin/sugar kinase superfamily. Four negative regulatory factors, Lst8p, Mks1p, and two redundant 14-3-3 proteins, Bmh1/2p, function between Rtg2p and Rtg1/3p. Alternative interaction between Mks1p and Rtg2p or Bmh1/2p provides a means for regulation of the RTG pathway. When the RTG pathway is on, Mks1p is inactivated by its association with Rtg2p; and when the RTG pathway is off, Mks1p dissociates from Rtg2p and forms a complex with Bmh1/2p, which is the negative regulatory form of Mks1p. Here we show that Rtg2p and Mks1p can interact in the absence of other factors, and is thereby the minimal binary switch for regulation of the RTG pathway. Gel filtration experiments indicate that both Rtg2p and Mks1p exist in high molecular weight complexes. In response to changes in the activity of the RTG pathway, both Rtg2p and Mks1p shift to different sized high molecular weight complexes. Together, our data suggest that dynamic association between Mks1p and Rtg2p in high molecular weight complexes provides a means to regulate the RTG pathway.
ER  -

ŠPÍREK, Mário, JR FERREIRA JÚNIOR a Ronald BUTOW. Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae. \textit{Gene}. Elsevier, 2005, roč.~354, jul 18, s.~2-8, 8 s. ISSN~0378-1119.

Podrobný výpis o publikaci