Low antigen-dependent activity of T cells after repeated
stimulation using dendritic cells and expansion with
interleukin-2.

J 2003

Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2.

BÜCHLER, Tomáš, Roman HÁJEK, Lucie KOVÁŘOVÁ, R. MUSILOVÁ, L. BOURKOVÁ et. al.

Basic information

Original name

Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2.

Name in Czech

Nízká na antigenu závislá aktivita T buněk po opakované stimulaci dendritickými buňkami a expanzi s IL-2

Authors

BÜCHLER, Tomáš (703 Slovakia), Roman HÁJEK (203 Czech Republic, guarantor), Lucie KOVÁŘOVÁ (203 Czech Republic), R. MUSILOVÁ (203 Czech Republic), L. BOURKOVÁ (203 Czech Republic), Zbyněk ČECH (203 Czech Republic), P. VÁNOVÁ (203 Czech Republic), L. TŮZOVÁ (203 Czech Republic), Petra VIDLÁKOVÁ (203 Czech Republic), Jiří VORLÍČEK (203 Czech Republic) and Miroslav PENKA (203 Czech Republic)

Edition

Neoplasma, Slovakia, Slovak Academic Press Ltd. 2003, 0028-2685

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.782

Organization unit

Faculty of Medicine

UT WoS

000186334800006

Keywords (in Czech)

dendritická buňka;T-lymfocyt;interleukin-2

Keywords in English

dendritic cell; IL-2;T cell

Abstract

ORIG CZ

V originále

Both CD8+ and CD4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation and proliferation of T cells require cellular interactions including adhesion, recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). DCs were obtained from peripheral blood mononuclear cells (PBMC) in the presence of IL-4 and GM-CSF. TNF-alpha was added to induce maturation. A conjugate of myeloma idiotypic protein with keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of II-2 and IL-7. Autologous DCs were added to the lymphocyte cultures on days 3, 10, and 17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in response to antigen-loaded DC but failed to do so if less than 0.3x10(6) DCs were added for stimulation during culture. However, no cytotoxic activity against autologous DCs was detected and IFN-gamma production in the T cell cultures was low at the end of culture. In conclusion, the generation and expansion of T cells using repeated stimulation by autologous DCs is feasible but defective cytotoxic reponse of these cells occurs, possibly as a consequence of repeated frequent exposure to antigen.

In Czech

V publikaci je popsána aktivita T lymfocytů po expozici dendritickými buňkami a expanzi s interleukinem-2.

Citovat

BÜCHLER, Tomáš, Roman HÁJEK, Lucie KOVÁŘOVÁ, R. MUSILOVÁ, L. BOURKOVÁ, Zbyněk ČECH, P. VÁNOVÁ, L. TŮZOVÁ, Petra VIDLÁKOVÁ, Jiří VORLÍČEK and Miroslav PENKA. Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2. Neoplasma. Slovakia: Slovak Academic Press Ltd., 2003, vol. 50, No 5, p. 345-349. ISSN 0028-2685.

@article{838007,
   author = {Büchler, Tomáš and Hájek, Roman and Kovářová, Lucie and Musilová, R. and Bourková, L. and Čech, Zbyněk and Vánová, P. and Tůzová, L. and Vidláková, Petra and Vorlíček, Jiří and Penka, Miroslav},
   article_location = {Slovakia},
   article_number = {5},
   keywords = {dendritic cell; IL-2;T cell},
   language = {eng},
   issn = {0028-2685},
   journal = {Neoplasma},
   title = {Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2.},
   volume = {50},
   year = {2003}
}

TY  - JOUR
ID  - 838007
AU  - Büchler, Tomáš - Hájek, Roman - Kovářová, Lucie - Musilová, R. - Bourková, L. - Čech, Zbyněk - Vánová, P. - Tůzová, L. - Vidláková, Petra - Vorlíček, Jiří - Penka, Miroslav
PY  - 2003
TI  - Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2.
JF  - Neoplasma
VL  - 50
IS  - 5
SP  - 345-349
EP  - 345-349
PB  - Slovak Academic Press Ltd.
SN  - 00282685
KW  - dendritic cell
KW  - IL-2;T cell
N2  - Both CD8+ and CD4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation and proliferation of T cells require cellular interactions including adhesion, recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). DCs were obtained from peripheral blood mononuclear cells (PBMC) in the presence of IL-4 and GM-CSF. TNF-alpha was added to induce maturation. A conjugate of myeloma idiotypic protein with keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of II-2 and IL-7. Autologous DCs were added to the lymphocyte cultures on days 3, 10, and 17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in response to antigen-loaded DC but failed to do so if less than 0.3x10(6) DCs were added for stimulation during culture. However, no cytotoxic activity against autologous DCs was detected and IFN-gamma production in the T cell cultures was low at the end of culture. In conclusion, the generation and expansion of T cells using repeated stimulation by autologous DCs is feasible but defective cytotoxic reponse of these cells occurs, possibly as a consequence of repeated frequent exposure to antigen.
ER  -

BÜCHLER, Tomáš, Roman HÁJEK, Lucie KOVÁŘOVÁ, R. MUSILOVÁ, L. BOURKOVÁ, Zbyněk ČECH, P. VÁNOVÁ, L. TŮZOVÁ, Petra VIDLÁKOVÁ, Jiří VORLÍČEK and Miroslav PENKA. Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2. \textit{Neoplasma}. Slovakia: Slovak Academic Press Ltd., 2003, vol.~50, No~5, p.~345-349. ISSN~0028-2685.

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