ADAM, Zdeněk, Martin KLABUSAY, Jiří VORLÍČEK and Roman HÁJEK. Detekce myelomových buněk v periferní krvi pomocí průtokové cytometrie (Detection of myeloma cells in the peripheral blood using flow cytometry). Vnitřní lékařství. Praha: Česk lékařská společnost J. Ev. Purkyně, 1997, vol. 43, No 9, p. 592-598. ISSN 0042-773X.
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Basic information
Original name Detekce myelomových buněk v periferní krvi pomocí průtokové cytometrie
Name (in English) Detection of myeloma cells in the peripheral blood using flow cytometry
Authors ADAM, Zdeněk (203 Czech Republic, guarantor), Martin KLABUSAY (203 Czech Republic), Jiří VORLÍČEK (203 Czech Republic) and Roman HÁJEK (203 Czech Republic).
Edition Vnitřní lékařství, Praha, Česk lékařská společnost J. Ev. Purkyně, 1997, 0042-773X.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Medicine
Keywords (in Czech) mnohočetný myelom
Keywords in English multiple myeloma; flow cytometry
Tags flow cytometry, multiple myeloma
Changed by Changed by: Mgr. Anna Potáčová, Ph.D., učo 44190. Changed: 23/6/2009 17:51.
Abstract
V publikaci je popsána možnost detekce myelomových buněk v periferní krvi pomocí průtokové cytometrie.
Abstract (in English)
Bone marrow plasma cells from patients with multiple myeloma express monoclonal cytoplasmic immunoglobulin, strongly express CD38 and usually coexpress CD56 and CD54. The aim of this study was to learn the relationship between the number of CD38+CD56+ and CD38+CD54 positive cells in peripheral blood with the activity of multiple myeloma. We evaluated the number of these cells in patients with monoclonal gammopathy and in the control group, as well. We used the two-color flow cytometry for this purpose. The peripheral blood of 57 patients with multiple myeloma and 4 patients with monoclonal gammopathy of unknown significance was repeatedly analyzed. Patients with high activity of multiple myeloma had the highest percentage of CD38+CD56+ and CD38+CD54+ cells. Patients with lower activity of the disease had lower count of these cells. But in 4 (11%) patients with high disease activity the count of CD38+CD56+ cells was zero. We conclude, that the longitudinal flow cytometric analysis of CD38+CD54+ and CD38+CD56+ cells is a useful method for estimation of disease activity in patients with multiple myeloma. Single analysis of these parameters cannot be used as a strict criterium for differential diagnosis.
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