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@inproceedings{839526, author = {Kristová, Petra and Hégrová, Blanka and Farková, Marta and Preisler, Jan and Lubal, Přemysl and Trnková, Libuše}, address = {Brno}, booktitle = {IX. Pracovní setkání fyzikálních chemiků a elektrochemiků}, edition = {2009}, keywords = {purine bases - HPLC - pKa values}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Brno}, isbn = {978-80-7375-309-2}, pages = {49-50}, publisher = {MZLU, Brno}, title = {Purine derivates - separation and characterization by selected methods}, year = {2009} }
TY - JOUR ID - 839526 AU - Kristová, Petra - Hégrová, Blanka - Farková, Marta - Preisler, Jan - Lubal, Přemysl - Trnková, Libuše PY - 2009 TI - Purine derivates - separation and characterization by selected methods PB - MZLU, Brno CY - Brno SN - 9788073753092 KW - purine bases - HPLC - pKa values N2 - Purine bases represent the basic constituents of DNA, their analysis is useful in the diagnosis of several diseases and metabolite disorders. The related bases, such as 6-aminopurine (6-AP, 2-aminopurine (2-AP) and 2,6-diaminopurine (2,6-DAP) were studies by spectroscopic and RP-HPLC techniques. Studied APs can be separated based on their spectral properties. Elution order related to polarity of molecule, in MF MetOH/30mM phosphate buffer (15:85), was following: 2,6-DAP, 2-AP, 6-AP. The phosphate concentration is not crucial. Experimental pKa values of model APs were calculated from corrected capacity factor dependence of APs on pH. The basicity increasing in order 2-AP<6-AP<2,6-DAP. Studies of basic structures will be used for nucleosides and nucleotides study, where 2-AP and 2,6-DAP can be used as fluorescent labels. ER -
KRISTOVÁ, Petra, Blanka HÉGROVÁ, Marta FARKOVÁ, Jan PREISLER, Přemysl LUBAL and Libuše TRNKOVÁ. Purine derivates - separation and characterization by selected methods. In \textit{IX. Pracovní setkání fyzikálních chemiků a elektrochemiků}. 2009th ed. Brno: MZLU, Brno, 2009, p.~49-50. ISBN~978-80-7375-309-2.
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