PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR and Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, vol. 394, No 5, p. 1497-1504. ISSN 1618-2642. |
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@article{839617, author = {Pekarová, Michaela and Moravcová, Jana and Kubala, Lukáš and Číž, Milan and Lojek, Antonín and Gregor, Čeněk and Hrbáč, Jan}, article_location = {HEIDELBERG, GERMANY}, article_number = {5}, keywords = {Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression}, language = {eng}, issn = {1618-2642}, journal = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY}, title = {Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7}, volume = {394}, year = {2009} }
TY - JOUR ID - 839617 AU - Pekarová, Michaela - Moravcová, Jana - Kubala, Lukáš - Číž, Milan - Lojek, Antonín - Gregor, Čeněk - Hrbáč, Jan PY - 2009 TI - Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7 JF - ANALYTICAL AND BIOANALYTICAL CHEMISTRY VL - 394 IS - 5 SP - 1497-1504 EP - 1497-1504 PB - SPRINGER-VERLAG HEIDELBERG SN - 16182642 KW - Nitric oxide KW - Macrophages KW - RAW 264.7 KW - Nitric oxide sensor KW - Nitrites KW - iNOS expression N2 - In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment. ER -
PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR and Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. \textit{ANALYTICAL AND BIOANALYTICAL CHEMISTRY}. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, vol.~394, No~5, p.~1497-1504. ISSN~1618-2642.
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