PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR and Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, vol. 394, No 5, p. 1497-1504. ISSN 1618-2642.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7
Name in Czech Měření produkce oxidu dusnatého u myších makrofágů RAW 264.7
Authors PEKAROVÁ, Michaela (703 Slovakia, guarantor), Jana MORAVCOVÁ (203 Czech Republic), Lukáš KUBALA (203 Czech Republic), Milan ČÍŽ (203 Czech Republic), Antonín LOJEK (203 Czech Republic), Čeněk GREGOR (203 Czech Republic) and Jan HRBÁČ (203 Czech Republic).
Edition ANALYTICAL AND BIOANALYTICAL CHEMISTRY, HEIDELBERG, GERMANY, SPRINGER-VERLAG HEIDELBERG, 2009, 1618-2642.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.480
RIV identification code RIV/00216224:14310/09:00028535
Organization unit Faculty of Science
UT WoS 000266918600029
Keywords (in Czech) oxid dusnatý, RAW 264.7, iNOS exprese, senzor pro oxid dusnatý
Keywords in English Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression
Tags iNOS expression, macrophages, nitric oxide, Nitric oxide sensor, Nitrites, RAW 264.7
Tags International impact
Changed by Changed by: Mgr. Michaela Pekarová, Ph.D., učo 85246. Changed: 26/6/2010 13:16.
Abstract
In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment.
Abstract (in Czech)
V této studii jsme se zaměřili na jednu z přímých metod stanovení produkce oxidu dusnatého v biologických systémech. Bylo realizováno dlouhodobé a kontinuální elektrochemické měření pomocí modifikovaného mikrosenzoru u makrofágů RAW 264.7.
Links
1QS500040507, research and development projectName: Tukové složky výživy - modulace jejich účinků a možnosti praktického využití
PrintDisplayed: 13/6/2024 20:40