Analysis of significant surface structures of selected parasite
taxa using the scanning eletron microscopy

a 2009

Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

HODOVÁ, Iveta

Základní údaje

Originální název

Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

Autoři

HODOVÁ, Iveta (203 Česká republika, garant)

Vydání

Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia), 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/09:00029407

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7399-739-7

Klíčová slova anglicky

SEM;Monogenea;Nematoda

Štítky

Monogenea, Nematoda, SEM

Změněno: 9. 2. 2010 16:25, Mgr. Iveta Hodová, Ph.D.

Anotace

V originále

Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.

Návaznosti

GA524/07/1610, projekt VaV

Název: Plasticita fenotypu monogeneí: kombinace molekulárního a morfologického přístupu

Investor: Grantová agentura ČR, Plasticita fenotypu monogeneí: kombinace molekulárního a morfologického přístupu

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

HODOVÁ, Iveta. Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy. In Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia). 2009. ISBN 978-80-7399-739-7.

@proceedings{840088,
   author = {Hodová, Iveta},
   booktitle = {Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia)},
   keywords = {SEM;Monogenea;Nematoda},
   language = {eng},
   isbn = {978-80-7399-739-7},
   title = {Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy},
   year = {2009}
}

TY  - CONF
ID  - 840088
AU  - Hodová, Iveta
PY  - 2009
TI  - Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy
SN  - 9788073997397
KW  - SEM;Monogenea;Nematoda
N2  - Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.
ER  -

HODOVÁ, Iveta. Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy. In \textit{Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia)}. 2009. ISBN~978-80-7399-739-7.

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