Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

HODOVÁ, Iveta. Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy. In Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia). 2009. ISBN 978-80-7399-739-7.

Basic information

• Original name: Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy
• Authors: HODOVÁ, Iveta (203 Czech Republic, guarantor).
• Edition: Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia), 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Slovakia
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/09:00029407
• Organization unit: Faculty of Science
• ISBN: 978-80-7399-739-7
• Keywords in English: SEM;Monogenea;Nematoda
• Tags: Monogenea, Nematoda, SEM

Abstract

Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.

Links

• GA524/07/1610, research and development project: Name: Plasticita fenotypu monogeneí: kombinace molekulárního a morfologického přístupu

Investor: Czech Science Foundation, Phenotypic plasticity of monogenean parasites: a combined molecular and morphological approach

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time