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@article{840541,
author = {Húska, Dalibor and Adam, Vojtěch and Trnková, Libuše and Kizek, René},
article_number = {10},
keywords = {Paramagnetic particles; Adenine; Adsorptive transfer stripping technique; Square wave voltammetry},
language = {eng},
issn = {0304-8853},
journal = {JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS},
title = {Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements},
volume = {321},
year = {2009}
}
TY - JOUR
ID - 840541
AU - Húska, Dalibor - Adam, Vojtěch - Trnková, Libuše - Kizek, René
PY - 2009
TI - Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements
JF - JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
VL - 321
IS - 10
SP - 1474-1477
EP - 1474-1477
SN - 03048853
KW - Paramagnetic particles
KW - Adenine
KW - Adsorptive transfer stripping technique
KW - Square wave voltammetry
N2 - The main aim of this work was to study the dependence of oligo adenine isolation efficiency on the chain length by using paramagnetic particles covered by homo eoxythymidines ((dT)25) with subsequent detection by adsorptive transfer technique coupled with square wave voltammetry. For this purpose, the oligonucleotides of the length A5, A10, A15, A20, A25, A30, A35, A40 and poly(A) in various concentrations were chosen. We determined that the isolation efficiency defined as "isolated oligonucleotide concentration''/"given oligonucleotide concentration" was about 55% on average. Sequence A25 demonstrated the best binding onto microparticles surface. (c) 2009 Elsevier B.V. All rights reserved.
ER -
HÚSKA, Dalibor, Vojtěch ADAM, Libuše TRNKOVÁ a René KIZEK. Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements. \textit{JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS}. 2009, roč.~321, č.~10, s.~1474-1477. ISSN~0304-8853.
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