2009
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
VALIGUROVÁ, Andrea, Veronika MICHALKOVÁ a Břetislav KOUDELAZákladní údaje
Originální název
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
Název česky
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
Autoři
VALIGUROVÁ, Andrea (703 Slovensko, garant, domácí), Veronika MICHALKOVÁ (703 Slovensko, domácí) a Břetislav KOUDELA (203 Česká republika)
Vydání
International Journal for Parasitology, Australie, Elsevier Science, 2009, 0020-7519
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Austrálie
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.819
Kód RIV
RIV/00216224:14310/09:00036268
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000269115000007
Klíčová slova anglicky
Epimerite retraction; Epicellular localisation; Eugregarine trophozoite; Actin; Myosin; Gregarina polymorpha
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 18. 3. 2011 16:55, doc. RNDr. Andrea Bardůnek Valigurová, Ph.D.
V originále
Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.
Česky
Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.
Návaznosti
| LC522, projekt VaV |
| ||
| MSM0021622416, záměr |
|