CHEMANI, Chanez, Anne IMBERTY, Sophie DE BENTZMANN, Maud PIERRE, Michaela WIMMEROVÁ, Benoit P. GUERY a Karine FAURE. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands. INFECTION AND IMMUNITY. The American Society for Microbiology, 2009, roč. 77, č. 5, s. 2065-2075. ISSN 0019-9567. |
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@article{842446, author = {Chemani, Chanez and Imberty, Anne and de Bentzmann, Sophie and Pierre, Maud and Wimmerová, Michaela and Guery, Benoit P. and Faure, Karine}, article_number = {5}, keywords = {lectin; infection;preudomonas aeruginosa}, language = {cze}, issn = {0019-9567}, journal = {INFECTION AND IMMUNITY}, title = {Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands}, volume = {77}, year = {2009} }
TY - JOUR ID - 842446 AU - Chemani, Chanez - Imberty, Anne - de Bentzmann, Sophie - Pierre, Maud - Wimmerová, Michaela - Guery, Benoit P. - Faure, Karine PY - 2009 TI - Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands JF - INFECTION AND IMMUNITY VL - 77 IS - 5 SP - 2065-2075 EP - 2065-2075 PB - The American Society for Microbiology SN - 00199567 KW - lectin KW - infection;preudomonas aeruginosa N2 - Pseudomonas aeruginosa is a frequently encountered pathogen that is involved in acute and chronic lung infections. Lectin-mediated bacterium-cell recognition and adhesion are critical steps in initiating P. aeruginosa pathogenesis. This study was designed to evaluate the contributions of LecA and LecB to the pathogenesis of P. aeruginosa-mediated acute lung injury. Using an in vitro model with A549 cells and an experimental in vivo murine model of acute lung injury, we compared the parental strain to lecA and lecB mutants. The effects of both LecA- and Lec B-specific lectin-inhibiting carbohydrates (a-methyl-galactoside and a-methyl-fucoside, respectively) were evaluated. In vitro, the parental strain was associated with increased cytotoxicity and adhesion on A549 cells compared to the lecA and lecB mutants. In vivo, the P. aeruginosa-induced increase in alveolar barrier permeability was reduced with both mutants. The bacterial burden and dissemination were decreased for both mutants compared with the parental strain. Coadministration of specific lectin inhibitors markedly reduced lung injury and mortality. Our results demonstrate that there is a relationship between lectins and the pathogenicity of P. aeruginosa ER -
CHEMANI, Chanez, Anne IMBERTY, Sophie DE BENTZMANN, Maud PIERRE, Michaela WIMMEROVÁ, Benoit P. GUERY a Karine FAURE. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands. \textit{INFECTION AND IMMUNITY}. The American Society for Microbiology, 2009, roč.~77, č.~5, s.~2065-2075. ISSN~0019-9567.
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