Does amount of light harvesting complexes play a role in the
sensitivity of DCMU-treated Arabidopsis thaliana to high light
stress?

a 2009

Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?

ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ a Miloš BARTÁK

Základní údaje

Originální název

Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?

Název česky

Hraje množství světlosběrných komplexů roli v citlivosti rostlin A. thalina ovlivněných DCMU na stres vyokým zářením?

Autoři

ŠTEPIGOVÁ, Jana (703 Slovensko), Kristýna VEČEŘOVÁ (203 Česká republika) a Miloš BARTÁK (203 Česká republika, garant)

Vydání

7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009, 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/09:00029456

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7375-310-8

Klíčová slova česky

fotoinhibice; LHC mutanti; fotosystém II; stres; antioxidanty; fluorometrie

Klíčová slova anglicky

photoinhibition; LHC lacking mutants; photosystem II; stress; chlorophyll fluorescence

Změněno: 31. 3. 2010 14:54, prof. Ing. Miloš Barták, CSc.

Anotace

ORIG CZ

V originále

Several fluorometric techniques, such as chlorophyll(Chl) fluorescence imaging, saturation pulse method, fast Chl fluorescence transients (OJIPs) were used to study photoinhibition and recovery in potted plants of A. thaliana. To address the role of light harvesting complexes(LHCs), wild type (wt-Columbia), npq (defective in zeaxanthin deepoxidase), and lhc (40 % reduction in LHCs) mutants were used. Responses to short-term (2000 microE m-2 s-1 PAR for 30 min) and long-term (300 microE m-2 s-1 PAR for 30 min plus PS II blocker DCMU) treatments were measured. In short-term experiment, photoinhibition of PS II was apparent as a decrease in Chl fluorescence in OJIPs, PS II quantum yield, and Fv/Fm ratio. The parameters, however, showed rapid recovery after 180 min in dark indicating high capacity of photoprotective mechanisms. Timecourses of Chl fluorescence parameters differed between wt, npq and lhc plants. In long-term treatment, the rate of DCMU (applied into substrate) uptake and interaction with light differed between wt and lhc plants. The results indicated that LHC-deficient A. thaliana mutants were more sensitive to photoinhibition than wt control. Supported by GACR52206097.

Česky

Měkolik metod indukované fluorescence chlorofylu bylo využito při studiu projevů fotoinhibice ve fotosystému II A. thaliana, a to rostlin kontrolních (wt), mutantů deficientních zeaxantin deepoxidázou a mutantů s redukovaným množstvím světlosběrných komplexů (LHC).

Návaznosti

GA522/06/0979, projekt VaV

Název: Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu

Investor: Grantová agentura ČR, Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu

Citovat

ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ a Miloš BARTÁK. Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress? In 7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009. 2009. ISBN 978-80-7375-310-8.

@proceedings{842888,
   author = {Štepigová, Jana and Večeřová, Kristýna and Barták, Miloš},
   booktitle = {7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009},
   keywords = {photoinhibition; LHC lacking mutants; photosystem II; stress; chlorophyll fluorescence},
   language = {eng},
   isbn = {978-80-7375-310-8},
   title = {Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?},
   url = {http://phd.czweb.org/phd09/},
   year = {2009}
}

TY  - CONF
ID  - 842888
AU  - Štepigová, Jana - Večeřová, Kristýna - Barták, Miloš
PY  - 2009
TI  - Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?
SN  - 9788073753108
KW  - photoinhibition
KW  - LHC lacking mutants
KW  - photosystem II
KW  - stress
KW  - chlorophyll fluorescence
UR  - http://phd.czweb.org/phd09/
N2  - Several fluorometric techniques, such as chlorophyll(Chl) fluorescence imaging, saturation pulse method, fast Chl fluorescence transients (OJIPs) were used to study photoinhibition and recovery in potted plants of A. thaliana. To address the role of light harvesting complexes(LHCs), wild type (wt-Columbia), npq (defective in zeaxanthin deepoxidase), and lhc (40 % reduction in LHCs) mutants were used. Responses to short-term (2000 microE m-2 s-1 PAR for 30 min) and long-term (300 microE m-2 s-1 PAR for 30 min plus PS II blocker DCMU) treatments were measured. In short-term experiment, photoinhibition of PS II was apparent as a decrease in Chl fluorescence in OJIPs, PS II quantum yield, and Fv/Fm ratio. The parameters, however, showed rapid recovery after 180 min in dark indicating high capacity of photoprotective mechanisms. Timecourses of Chl fluorescence parameters differed between wt, npq and lhc plants. In long-term treatment, the rate of DCMU (applied into substrate) uptake and interaction with light differed between wt and lhc plants. The results indicated that LHC-deficient A. thaliana mutants were more sensitive to photoinhibition than wt control. Supported by GACR52206097.
ER  -

ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ a Miloš BARTÁK. Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress? In \textit{7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009}. 2009. ISBN~978-80-7375-310-8.

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