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@article{844818,
author = {Řepková, Jana and Hofbauer, Jan},
article_location = {Česká republika},
article_number = {2},
keywords = {interspecific hybridization; Lotus; pod shattering; post-fertilization barriers; pre-fertilization barriers},
language = {eng},
issn = {1212-1975},
journal = {Czech Journal of Genetics and Plant Breeding},
title = {Seed pod shattering in the genus Lotus and its overcoming},
volume = {45},
year = {2009}
}
TY - JOUR
ID - 844818
AU - Řepková, Jana - Hofbauer, Jan
PY - 2009
TI - Seed pod shattering in the genus Lotus and its overcoming
JF - Czech Journal of Genetics and Plant Breeding
VL - 45
IS - 2
SP - 39-44
EP - 39-44
PB - Czech Academy of Agrisultural Sciences
SN - 12121975
KW - interspecific hybridization
KW - Lotus
KW - pod shattering
KW - post-fertilization barriers
KW - pre-fertilization barriers
N2 - Birdsfoot trefoil (Lotus corniculatus L.) is a perennial species that is known for its outstanding characters as a crucial component of meadows and pasture vegetation and a highly successful fodder crop. However, its cultivation has been limited by the inability to control pod shattering. The anatomic and physiological bases of pod shattering are known and are considered to be controlled by more than one gene. This paper reviews the known causes of pod shattering and potential ways of overcoming pod indehiscence in L. corniculatus. Genetic transformation is possible in the genus Lotus; however, useful genes determining seed pod indehiscent character have not yet been identified. The only way of introducing pod indehiscence characters into L. corniculatus is by interspecific hybridization within the genus Lotus; embryo rescue and protoplast cultures are promising. To determine useful genotypes for crosses, investigations of pre- and post-fertilization barriers are necessary. To that end, we present here a convenient procedure for a whole-mount clearing treatment of immature seeds that leaves the cell walls of tissues intact. This is a useful technique for the study of post-fertilization barriers in Lotus.
ER -
ŘEPKOVÁ, Jana a Jan HOFBAUER. Seed pod shattering in the genus Lotus and its overcoming. \textit{Czech Journal of Genetics and Plant Breeding}. Česká republika: Czech Academy of Agrisultural Sciences, 2009, roč.~45, č.~2, s.~39-44. ISSN~1212-1975.
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