2009
Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples
PREISLER, Jan; Pavla JUNGOVÁ; Ondřej PEŠ; Tomáš VACULOVIČ; Jarmila NAVRÁTILOVÁ et. al.Basic information
Original name
Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples
Name in Czech
Kombinace hmotnostní spektrometrie laserové desorpce s indukčně vázaným plazmatem pro elementální detekci mikrokolonových eluátů a biologických submikrolitrových vzorků
Authors
PREISLER, Jan (203 Czech Republic, guarantor, belonging to the institution); Pavla JUNGOVÁ (203 Czech Republic, belonging to the institution); Ondřej PEŠ (203 Czech Republic); Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution); Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution); Jan ŠMARDA (203 Czech Republic) and Viktor KANICKÝ (203 Czech Republic, belonging to the institution)
Edition
23rd International Symposium on MicroScale Bioseparations (MSB), 2009
Other information
Language
English
Type of outcome
Conference abstract
Field of Study
10406 Analytical chemistry
Country of publisher
United States of America
Confidentiality degree
is not subject to a state or trade secret
References:
RIV identification code
RIV/00216224:14310/09:00029473
Organization unit
Faculty of Science
Keywords in English
laser desorption; laser ablation; inductively coupled plasma; SALD; copper; submicroliter samples; myeloid leukemia cells
Tags
International impact, Reviewed
Changed: 9/4/2013 12:29, prof. Mgr. Jan Preisler, Ph.D.
In the original language
A new method of sample preparation for elemental analysis using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS) is presented. Well-defined submicroliter volumes of analyzed samples are deposited onto polyethylene terephthalate glycol (PETG) plate and, after insertion into a commercial ablation cell, desorbed with pulses of 213-nm laser and analyzed with ICP MS. In contrast to conventional laser ablation (LA) ICP MS of solids, the analyzed compound is quantitatively desorbed during the PETG ablation. Furthermore, complete desorption is achieved at laser power density as low as ~10 MW/cm2, two orders below the levels typical in LA ICP MS. Model samples of metal salts in pg quantities were desorbed by less than 20 laser shots and relative spot-to-spot reproducibility was 3-10%. The limits of detection of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the range of 50-500 fg. Selection of optimal conditions, such as laser fluence, number of laser shots, type of raster etc. will be discussed. The low limits of detection allow using SALD for off-line coupling of capillary electrophoresis (CE) to ICP MS.
In Czech
Viz anglický popis.
Links
| GA203/09/1025, research and development project |
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| MSM0021622411, plan (intention) |
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| MSM0021622412, plan (intention) |
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| MSM0021622415, plan (intention) |
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