a 2009

Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Ivo PROVAZNÍK and Marie NOVÁKOVÁ

Basic information

Original name

Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS

Name in Czech

Studium jevu zvaného preconditioning na modelu izolovaného srdce morčete a králíka pomocí napěťově citlivého barviva di-4-ANEPPS

Authors

FIALOVÁ, Kateřina (203 Czech Republic, guarantor), Jana KOLÁŘOVÁ (203 Czech Republic), Ivo PROVAZNÍK (203 Czech Republic) and Marie NOVÁKOVÁ (203 Czech Republic)

Edition

2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14110/09:00029526

Organization unit

Faculty of Medicine

UT WoS

000279344300133

Keywords (in Czech)

preconditioning; izolované srdce; napěťově citlivá barviva; ischemie; reperfůze

Keywords in English

preconditioning; isolated heart; voltage-sensitive dye; ischemia; reperfusion

Tags

International impact, Reviewed
Změněno: 1/9/2010 14:08, MUDr. Kateřina Fialová, Ph.D.

Abstract

ORIG CZ

V originále

Myocardial ischemia and reperfusion phenomena have been studied repeatedly in various models. The aim of this study was to verify preconditioning model in guinea pig and rabbit Langendorff-perfused hearts loaded with voltage-sensitive dye (VSD) di-4-ANEPPS which we use to record monophasic action potentials (MAPs) by optical method. The heart was perfused according to Langendorff at constant pressure (80mmHg) with Krebs Henseleit solution. Each experiment consisted of heart isolation, control perfusion, dye loading, washout and MAPs recording. Rabbit hearts loaded with VSD tolerated ischemia of longer duration without severe alterations of electrogram. Although the procedure of dye loading affects electrophysiological properties of the myocardium, in case of rabbit heart it is negligible (it is quite resistant to side effects of VSDs). In guinea pig mycardium, more cautiousness is needed.

In Czech

Práce se zabývá studiem fenoménu zvaného preconditioning u izolovaných srdcí morčete a králíka barvených napěťově citlivým barvivem di-4-ANEPPS.

Links

GA102/07/1473, research and development project
Name: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací
Investor: Czech Science Foundation
MSM0021622402, plan (intention)
Name: Časná diagnostika a léčba kardiovaskulárních chorob
Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases