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@article{849022,
author = {Stixová, Lenka and Procházková, Jiřina and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois},
article_number = {1-2},
keywords = {Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;},
language = {eng},
issn = {0300-8177},
journal = {Mol Cell Biochem},
title = {5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway},
volume = {330},
year = {2009}
}
TY - JOUR
ID - 849022
AU - Stixová, Lenka - Procházková, Jiřina - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois
PY - 2009
TI - 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway
JF - Mol Cell Biochem
VL - 330
IS - 1-2
SN - 03008177
KW - Differentiation
KW - 1alpha25 dihydroxyvitamin D3
KW - 5 lipoxygenase inhibitors
KW - p38 MAPK;
N2 - The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.
ER -
STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ a Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. \textit{Mol Cell Biochem}. 2009, roč.~330, 1-2, 10 s. ISSN~0300-8177.
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