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@article{849022, author = {Stixová, Lenka and Procházková, Jiřina and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois}, article_number = {1-2}, keywords = {Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;}, language = {eng}, issn = {0300-8177}, journal = {Mol Cell Biochem}, title = {5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway}, volume = {330}, year = {2009} }
TY - JOUR ID - 849022 AU - Stixová, Lenka - Procházková, Jiřina - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois PY - 2009 TI - 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway JF - Mol Cell Biochem VL - 330 IS - 1-2 SN - 03008177 KW - Differentiation KW - 1alpha25 dihydroxyvitamin D3 KW - 5 lipoxygenase inhibitors KW - p38 MAPK; N2 - The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation. ER -
STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ a Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. \textit{Mol Cell Biochem}. 2009, roč.~330, 1-2, 10 s. ISSN~0300-8177.
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