| MATEJKOVÁ, Michaela, Jitka ŽÍDKOVÁ, Lukáš ŽÍDEK, Michaela WIMMEROVÁ, Josef CHMELÍK a Vladimír SKLENÁŘ. Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry. Journal of Agricultural and Food Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2009, roč. 57, č. 18, s. 8444-8452. ISSN 0021-8561. |
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@article{849041,
author = {Matejková, Michaela and Žídková, Jitka and Žídek, Lukáš and Wimmerová, Michaela and Chmelík, Josef and Sklenář, Vladimír},
article_location = {WASHINGTON},
article_number = {18},
keywords = {Non-specific lipid transfer protein 1; barley grain; thermaldenaturation; nuclear magnetic resonance; differential scanningcalorimetry},
language = {eng},
issn = {0021-8561},
journal = {Journal of Agricultural and Food Chemistry},
title = {Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry},
volume = {57},
year = {2009}
}
TY - JOUR
ID - 849041
AU - Matejková, Michaela - Žídková, Jitka - Žídek, Lukáš - Wimmerová, Michaela - Chmelík, Josef - Sklenář, Vladimír
PY - 2009
TI - Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry
JF - Journal of Agricultural and Food Chemistry
VL - 57
IS - 18
SP - 8444-8452
EP - 8444-8452
PB - AMER CHEMICAL SOC
SN - 00218561
KW - Non-specific lipid transfer protein 1
KW - barley grain
KW - thermaldenaturation
KW - nuclear magnetic resonance
KW - differential scanningcalorimetry
N2 - Process of thermal denaturation of covalently modified form of barley grain non-specific lipid transfer protein 1b (ns-LTP1b) was investigated by nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) up to 115\,$^\circ$C. The denaturation was found to be irreversible and highly cooperative. A method of numerical quantitative analysis allowing to fit the NMR data to a transition-state model without further simplification was developed. Based on the obtained values of transition state enthalpy and entropy, rate of denaturation was calculated as a simple measure of protein stability at various temperatures. Effect of disulfide bond reduction on thermal denaturation of ns-LTP1b was studied and discussed in the context of quality control of barley products during storage and processing.
ER -
MATEJKOVÁ, Michaela, Jitka ŽÍDKOVÁ, Lukáš ŽÍDEK, Michaela WIMMEROVÁ, Josef CHMELÍK a Vladimír SKLENÁŘ. Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry. \textit{Journal of Agricultural and Food Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2009, roč.~57, č.~18, s.~8444-8452. ISSN~0021-8561.
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