DYČKA, Filip, Markéta LAŠTOVIČKOVÁ, Martin ČERNÝ, Břetislav BRZOBOHATÝ and Janette BOBÁLOVÁ. Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In 3rd Central and Eastern European Proteomics Conference. Book of Abstracts. Budapest (HU), 2009, p. 104. ISBN 978-963-9319-99-8.
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Basic information
Original name Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Name in Czech Analýza fosfopeptidů s využitím obohacovacích technik a MALDI-TOF MS
Authors DYČKA, Filip, Markéta LAŠTOVIČKOVÁ, Martin ČERNÝ, Břetislav BRZOBOHATÝ and Janette BOBÁLOVÁ.
Edition Budapest (HU), 3rd Central and Eastern European Proteomics Conference. Book of Abstracts, p. 104-104, 2009.
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Hungary
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Science
ISBN 978-963-9319-99-8
Keywords in English proteomic analysis; phosphoproteome
Tags MALDI, mass spectrometry, TOF
Changed by Changed by: Mgr. Filip Dyčka, Ph.D., učo 77671. Changed: 13/10/2009 11:58.
Abstract
In the present study, two methods for enrichment of tryptic phosphopeptides were described: IMAC and titanium dioxide chromatography. After enrichment procedure the phosphopeptides were analyzed by MALDI-TOF MS. Alpha-casein was used as model phosphoprotein. The methods were applied to protein mixture of Arabidopsis thaliana.
Abstract (in Czech)
Práce se zabývá použitím obohacovacích technik, jako je IMAC a chromatografie s oxidem titaničitým, při analýze fosfopeptidů pomocí MALDI-TOF MS. Jako modelový fosfoprotein byl použit alfa-kasein. Metody bylo využito i při analýze proteinů z rostliny Arabidopsis.
Links
AV0Z40310501, plan (intention)Name: Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie
IAA600040701, research and development projectName: Dynamika proteomu v odpovědi na zvýšené hladiny cytokininů u Arabidopsis
Investor: Academy of Sciences of the Czech Republic, Proteome dynamics in response to increased cytokinin levels in Arabidopsis
1M06030, research and development projectName: Funkční genomika a proteomika ve šlechtění rostlin
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