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@misc{849963,
author = {Vařecha, Miroslav and Amrichová, Jana and Matula, Pavel and Kozubek, Michal},
booktitle = {Funkční organizace buněčného jádra},
keywords = {fluorescence microscopy; spinning disk; image nalysis},
language = {eng},
title = {Automated spinning disk confocal microscopy in 3D live cell imaging},
year = {2009}
}
TY - SLIDE
ID - 849963
AU - Vařecha, Miroslav - Amrichová, Jana - Matula, Pavel - Kozubek, Michal
PY - 2009
TI - Automated spinning disk confocal microscopy in 3D live cell imaging
KW - fluorescence microscopy
KW - spinning disk
KW - image nalysis
N2 - Fluorescence microscopy has become the leading technology to study structure and dynamics of cellular components and processes. The studies can be performed in two-dimensional (2D) but also in three-dimensional (3D) spatial coordinate system as well as in time and spectral dimensions. Fluorescent proteins allow us to study protein dynamics, localization, and interactions in living cells. In our laboratory, we have been developing special systems for automated cell image acquisition and analysis using fluorescence microscopy working up to five dimensions (x, y, z, t, lambda), whose hardware and software was optimized for studies on living cells. The presentation will focus on the latest developments in our technology. For the first time, we discovered interaction of apoptotic proteins AIF and endonuclease G, expressed using one DNA plasmid, in living human cells during apoptotic cell death.
ER -
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Pavel MATULA and Michal KOZUBEK. Automated spinning disk confocal microscopy in 3D live cell imaging. In \textit{Funkční organizace buněčného jádra}. 2009.
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