Detection of metallothionein and alpha-methylacyl CoA racemase
as potential new markers for prostate carcinoma

J 2009

Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma

MASAŘÍK, Michal, Jaromír GUMULEC, Šárka KUCHTÍČKOVÁ, Vojtěch ADAM, Soňa KŘÍŽKOVÁ et. al.

Basic information

Original name

Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma

Name in Czech

Detekce metalothioneinu a alpha-methylacyl CoA racemázy jako potenciálních markerů karcinomu prostaty

Authors

MASAŘÍK, Michal, Jaromír GUMULEC, Šárka KUCHTÍČKOVÁ, Vojtěch ADAM, Soňa KŘÍŽKOVÁ and René KIZEK

Edition

International Journal of Molecular Medicine, 2009, 1107-3756

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Greece

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.980

Organization unit

Faculty of Medicine

UT WoS

000270497500177

Keywords in English

prostate carcinoma; tumor markers; metallothionein; AMACR

Abstract

ORIG CZ

V originále

In many developed countries, prostate cancer is the second leading cause of cancer-related deaths among men. Therefore, having reliable and easy-to-detect marker is needed to treat this disease successfully. Metallothioneins (MT) are a group of proteins rich in cysteine with molecular weights ranging from 6 to 10 kDa. Their main physiological functions are homeostatic control and detoxification of the metals. The role of MT in tumour tissue remains still unclear, but MT can be considered as new promising tumour marker. Besides MT, alpha-methylacyl CoA racemase AMACR has been proven to be one of the few biomarkers that can help distinguish cancer from benign cells, with high sensitivity and specificity for prostate carcinoma. In this study we proposed new methodical approaches in MT and AMACR detection in cell line model and then reciprocal correlation of obtained data. The LNCaP cell line used in our experiments was isolated in 1977 by J.S. Horoszewicz, et al. An adsorptive transfer stripping technique coupled with differential pulsed voltammetry Brdicka reaction was employed for the determination of metallothionein MT and AMACR level in cell lines extract. MT content was hundred of ug per g of soluble proteins and was higher as well as for AMACR compared to non-tumour cell line.

In Czech

Práce popisuje detekci metalothioneinu a alpha-methylacyl CoA racemázu jako možných markerů pro diagnostiku karcinomu prostaty. Detekce byla prováděna standardními molekulárně-biologickými technikami a elektrochemickým přístupem.

Links

GP301/09/P436, research and development project

Name: Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu.

Investor: Czech Science Foundation

Citovat

MASAŘÍK, Michal, Jaromír GUMULEC, Šárka KUCHTÍČKOVÁ, Vojtěch ADAM, Soňa KŘÍŽKOVÁ and René KIZEK. Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma. International Journal of Molecular Medicine. 2009, vol. 24, Suppl. 1, 83 pp. ISSN 1107-3756.

@article{851149,
   author = {Masařík, Michal and Gumulec, Jaromír and Kuchtíčková, Šárka and Adam, Vojtěch and Křížková, Soňa and Kizek, René},
   article_number = {Suppl. 1},
   keywords = {prostate carcinoma; tumor markers; metallothionein; AMACR},
   language = {eng},
   issn = {1107-3756},
   journal = {International Journal of Molecular Medicine},
   title = {Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma},
   volume = {24},
   year = {2009}
}

TY  - JOUR
ID  - 851149
AU  - Masařík, Michal - Gumulec, Jaromír - Kuchtíčková, Šárka - Adam, Vojtěch - Křížková, Soňa - Kizek, René
PY  - 2009
TI  - Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma
JF  - International Journal of Molecular Medicine
VL  - 24
IS  - Suppl. 1
SN  - 11073756
KW  - prostate carcinoma
KW  - tumor markers
KW  - metallothionein
KW  - AMACR
N2  - In many developed countries, prostate cancer is the second leading cause of cancer-related deaths among men. Therefore, having reliable and easy-to-detect marker is needed to treat this disease successfully. Metallothioneins (MT) are a group of proteins rich in cysteine with molecular weights ranging from 6 to 10 kDa. Their main physiological functions are homeostatic control and detoxification of the metals. The role of MT in tumour tissue remains still unclear, but MT can be considered as new promising tumour marker. Besides MT, alpha-methylacyl CoA racemase AMACR has been proven to be one of the few biomarkers that can help distinguish cancer from benign cells, with high sensitivity and specificity for prostate carcinoma. In this study we proposed new methodical approaches in MT and AMACR detection in cell line model and then reciprocal correlation of obtained data. The LNCaP cell line used in our experiments was isolated in 1977 by J.S. Horoszewicz, et al. An adsorptive transfer stripping technique coupled with differential pulsed voltammetry Brdicka reaction was employed for the determination of metallothionein MT and AMACR level in cell lines extract. MT content was hundred of ug per g of soluble proteins and was higher as well as for AMACR compared to non-tumour cell line.
ER  -

MASAŘÍK, Michal, Jaromír GUMULEC, Šárka KUCHTÍČKOVÁ, Vojtěch ADAM, Soňa KŘÍŽKOVÁ and René KIZEK. Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma. \textit{International Journal of Molecular Medicine}. 2009, vol.~24, Suppl. 1, 83 pp. ISSN~1107-3756.

Detailed Information on Publication Record