2009
In vitro activation of cytotoxic T-lymphocytes by hTERT-pulsed dendritic cells
KRYUKOV, Fedor; Darina OČADLÍKOVÁ; Lucie KOVÁŘOVÁ; Ivana BUREŠOVÁ; Roman HÁJEK et. al.Basic information
Original name
In vitro activation of cytotoxic T-lymphocytes by hTERT-pulsed dendritic cells
Name in Czech
In vitro aktivace cytotoxických T-lymfocytů pomocí dendritických buněk naložených hTERT
Authors
KRYUKOV, Fedor; Darina OČADLÍKOVÁ; Lucie KOVÁŘOVÁ; Ivana BUREŠOVÁ; Roman HÁJEK and Jaroslav MICHÁLEK
Edition
Journal of immunotoxicology, England, Informa Healthcare, 2009, 1547-691X
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30200 3.2 Clinical medicine
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 1.218
RIV identification code
RIV/00216224:14110/09:00036980
Organization unit
Faculty of Medicine
UT WoS
000275001500006
Keywords (in Czech)
mnohočetný myelom; T-lymfocyt; hTERT
Keywords in English
multiple myeloma; T-lymphocyte; hTERT
Changed: 1/4/2010 16:42, Mgr. Anna Potáčová, Ph.D.
In the original language
In the studies reported here, we studied antigen-specific and HLA-A2-restricted cytotoxic activity against an ARH77 myeloma cell line in vitro. An HLA-A2-specific hTERT-derived nonapeptide ((540)ILAKFLHWL(548)) was used as a TAA. Myeloma-specific cytotoxic activity of hTERT-reactive cytotoxic lymphocytes (CTLs) was established by repeated stimulation of the CTLs via dendritic cells loaded with hTERT-derived nonapeptide. These studies were able to demonstrate that hTERT-reactive T-lymphocytes can be identified and expanded using relatively simple in vitro techniques consisting of antigen-specific stimulation, immunomagnetic sorting, and then induction of rapid expansion.
In Czech
V této práci jsou publikovány naše výsledky z in vitro studie, v níž byly měřeny antigen specifické a HLA-A2 omezené cytotoxické aktivity T-lymfocytů u linie ARH-77.
Links
| LC06027, research and development project |
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| 1A8709, research and development project |
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