Quantitative alterations of immunofluorescence staining for laminin-1 and matrix metalloproteinase-3 in spinal nerve roots of two neuropathic pain models

DAVID, Marek, Petr DUBOVÝ, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ. Quantitative alterations of immunofluorescence staining for laminin-1 and matrix metalloproteinase-3 in spinal nerve roots of two neuropathic pain models. In Morphology 2009 - Proceedings of the 45th International Congress on Anatomy and 46th Lojda Symposium on Histochemistry. 2009. ISBN 978-80-254-3882-4.

Základní údaje

• Originální název: Quantitative alterations of immunofluorescence staining for laminin-1 and matrix metalloproteinase-3 in spinal nerve roots of two neuropathic pain models
• Název česky: Kvantitativní změny imunofluorescenčního barvení lamininu 1 a matrix metaloproteinázy 3 v kořenech spinálního nervu u dvou modelů neuropatické bolesti
• Autoři: DAVID, Marek, Petr DUBOVÝ, Ilona KLUSÁKOVÁ a Ivana HRADILOVÁ SVÍŽENSKÁ.
• Vydání: Morphology 2009 - Proceedings of the 45th International Congress on Anatomy and 46th Lojda Symposium on Histochemistry, 2009.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 30000 3. Medical and Health Sciences
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• WWW: web stránky konference
• Organizační jednotka: Lékařská fakulta
• ISBN: 978-80-254-3882-4
• Klíčová slova česky: dorzální kořen; endoneurium; kořeny spinálního nervu; laminin 1; matrix metaloproteináza 3; neuropatická bolest; periferní nervová soustava; unilaterální poškození nervu; ventrální kořen
• Klíčová slova anglicky: dorsal root; endoneurium; laminin-1; matrix metalloproteinase-3; neuropathic pain; peripheral nervous system; spinal nerve roots; unilateral nerve injury; ventral root
• Štítky: dorsal root; endoneurium, Laminin-1, matrix metalloproteinase-3, neuropathic pain, peripheral nervous system, spinal nerve roots, unilateral nerve injury; ventral root

Anotace

Peripheral nerve injury leads to distal nerve stump degeneration. This process, which is called Wallerian degeneration, is accompanied by many cellular and molecular changes including myelin clearance and axonal breakdown. Events taking place in proximal nerve segments are less apparent and information about them in some compartments, such as spinal nerve roots, is very limited. Laminins, one group of extracellular matrix (ECM) molecules, are significantly involved in molecular processes related with regeneration of injured nerve. Matrix metalloproteinases are key enzymes influencing remodulation of extracellular matrix including laminins. We used quantitative immunohistochemical analysis of laminin-1 (lam-1) and matrix metalloproteinase-3 (MMP-3) in the spinal nerve roots following sciatic or spinal nerve ligature to confirm ECM alterations and its remodulation also more proximal to peripheral nerve injury. Compared to naive animals, a transient increase of lam-1 immunofluorescence (IF) intensity was observed in both ipsilateral and contralateral dorsal and ventral roots 1 and 3 days from sciatic or spinal nerve ligature. A bilateral decrease of lam-1 IF was detected in spinal nerve roots 7 or 14 days after both types of peripheral nerve injury. Brightness of MMP-3 IF gradually increased bilaterally in dorsal and ventral roots up to 3 (spinal nerve ligature) and 7 (sciatic nerve ligature) days from operation. A bilateral decrease of MMP-3 IF was found in roots during next monitored periods of survival - for spinal nerve ligature at 7 and for sciatic nerve ligature at 14 days following nerve injury. Alterations of IF were for both molecules generally more apparent in ipsilateral than contralateral roots. Larger changes of IF brightness were observed after sciatic than spinal nerve ligature. In conclusion, we demonstrated the remodulation of ECM in both ipsilateral and contralateral spinal nerve roots following unilateral peripheral nerve injury. In addition, we confirmed that matrix metalloproteinases are probably involved in this process.

Anotace česky

Peripheral nerve injury leads to distal nerve stump degeneration. This process, which is called Wallerian degeneration, is accompanied by many cellular and molecular changes including myelin clearance and axonal breakdown. Events taking place in proximal nerve segments are less apparent and information about them in some compartments, such as spinal nerve roots, is very limited. Laminins, one group of extracellular matrix (ECM) molecules, are significantly involved in molecular processes related with regeneration of injured nerve. Matrix metalloproteinases are key enzymes influencing remodulation of extracellular matrix including laminins. We used quantitative immunohistochemical analysis of laminin-1 (lam-1) and matrix metalloproteinase-3 (MMP-3) in the spinal nerve roots following sciatic or spinal nerve ligature to confirm ECM alterations and its remodulation also more proximal to peripheral nerve injury. Compared to naive animals, a transient increase of lam-1 immunofluorescence (IF) intensity was observed in both ipsilateral and contralateral dorsal and ventral roots 1 and 3 days from sciatic or spinal nerve ligature. A bilateral decrease of lam-1 IF was detected in spinal nerve roots 7 or 14 days after both types of peripheral nerve injury. Brightness of MMP-3 IF gradually increased bilaterally in dorsal and ventral roots up to 3 (spinal nerve ligature) and 7 (sciatic nerve ligature) days from operation. A bilateral decrease of MMP-3 IF was found in roots during next monitored periods of survival - for spinal nerve ligature at 7 and for sciatic nerve ligature at 14 days following nerve injury. Alterations of IF were for both molecules generally more apparent in ipsilateral than contralateral roots. Larger changes of IF brightness were observed after sciatic than spinal nerve ligature. In conclusion, we demonstrated the remodulation of ECM in both ipsilateral and contralateral spinal nerve roots following unilateral peripheral nerve injury. In addition, we confirmed that matrix metalloproteinases are probably involved in this process.

Návaznosti

• MSM0021622404, záměr: Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS