Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array

DUBOVÝ, Petr, Marek DAVID, Ivana HRADILOVÁ SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array. In 9th European Meeting on Glial Cells in Health and Disease. 2009. ISSN 0894-1491.

Basic information

Original name

Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array

Name in Czech

Bilaterální kvantitativní změny lamininu 1 v dorzálních a ventrálních kořenech po unilaterální chronické konstrikci sciatického nervu - Imunofluorescenční analýza tkáně

Authors

DUBOVÝ, Petr, Marek DAVID, Ivana HRADILOVÁ SVÍŽENSKÁ and Ilona KLUSÁKOVÁ

Edition

9th European Meeting on Glial Cells in Health and Disease, 2009

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

web stránky konference abstrakt v časopisu Glia - ISSN 0894-1491

Impact factor

Impact factor: 4.932

Organization unit

Faculty of Medicine

ISSN

UT WoS

000270075500609

Keywords (in Czech)

behaviorální testy; bilaterální reakce kořenů; dorzální kořen; endoneurium; kořeny spinálního nervu; laminin 1; neuropatická bolest; periferní nervová soustava; unilaterální konstrikce sciatického nervu; ventrální kořen

Keywords in English

behavioral tests; bilateral roots reaction; dorsal root; endoneurium; laminin-1; neuropathic pain; peripheral nervous system; spinal nerve roots; unilateral constriction of sciatic nerve; ventral root

Tags

behavioral tests, bilateral roots reaction, dorsal root, endoneurium, Laminin-1, neuropathic pain, peripheral nervous system, spinal nerve roots, unilateral constriction of sciatic nerve, ventral root

Tags

International impact

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Abstract

V originále

Bilateral changes of immunofluorescence for laminin-1 (lam-1) were investigated in the rat spinal nerve roots (L4-L5) following neuropathic pain induction by unilateral sciatic nerve ligature. Induction of neuropathic pain was confirmed in experimental animals by behavioral tests of mechanical allodynia and thermal hyperalgesia. Dorsal and ventral roots of naive rats displayed lam-1 immunostaining in the basal laminae on outer surface of Schwann cell/axon units, perineurial sheaths and blood vessels. Significantly higher brightness of immunofluorescence for lam-1 was observed in the ventral than dorsal roots removed from naive rats as well as those operated on sciatic nerve ligature and surviving for 1 or 3 days. Chronic constriction injury of sciatic nerve for 7 and 14 days results in reduction of different levels of immunofluorescence brightness between dorsal and ventral roots. In comparison to naive and sham-operated animals, apparent bilateral decrease of immunofluorescence brightness for lam-1 was found in both dorsal and ventral roots 7 days after unilateral nerve injury. Although the pattern of lam-1 alterations in both ipsi- and contralateral roots was similar, changes of immunofluorescence were more evident in ipsilateral than contralateral roots. The results indicate that quantitative changes of immunofluorescence for lam-1 are present bilaterally in proximal segments of peripheral nerve far from unilateral chronic constriction injury inducing neuropathic pain. This suggests a possible mechanism for misbalance alongside the afferent and motor axons, the involvement of which in neuropathic pain induction is assumed.

In Czech

Bilateral changes of immunofluorescence for laminin-1 (lam-1) were investigated in the rat spinal nerve roots (L4-L5) following neuropathic pain induction by unilateral sciatic nerve ligature. Induction of neuropathic pain was confirmed in experimental animals by behavioral tests of mechanical allodynia and thermal hyperalgesia. Dorsal and ventral roots of naive rats displayed lam-1 immunostaining in the basal laminae on outer surface of Schwann cell/axon units, perineurial sheaths and blood vessels. Significantly higher brightness of immunofluorescence for lam-1 was observed in the ventral than dorsal roots removed from naive rats as well as those operated on sciatic nerve ligature and surviving for 1 or 3 days. Chronic constriction injury of sciatic nerve for 7 and 14 days results in reduction of different levels of immunofluorescence brightness between dorsal and ventral roots. In comparison to naive and sham-operated animals, apparent bilateral decrease of immunofluorescence brightness for lam-1 was found in both dorsal and ventral roots 7 days after unilateral nerve injury. Although the pattern of lam-1 alterations in both ipsi- and contralateral roots was similar, changes of immunofluorescence were more evident in ipsilateral than contralateral roots. The results indicate that quantitative changes of immunofluorescence for lam-1 are present bilaterally in proximal segments of peripheral nerve far from unilateral chronic constriction injury inducing neuropathic pain. This suggests a possible mechanism for misbalance alongside the afferent and motor axons, the involvement of which in neuropathic pain induction is assumed.

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Links

MSM0021622404, plan (intention)

Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.