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@article{858028, author = {Vojtylová, Terézia and Dospivová, Dana and Třísková, Olga and Pilařová, Iveta and Lubal, Přemysl and Farková, Marta and Trnková, Libuše and Táborský, Petr}, article_number = {6}, keywords = {oligonucleotides; adenine; cytosine; protonation; thermodynamics; chemometrics}, language = {eng}, issn = {0366-6352}, journal = {Chem. Papers}, title = {Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine}, url = {http://www.springerlink.com/content/b163418v38432172/?p=afb907946c874228a3c45a57420400e5&pi=15}, volume = {63}, year = {2009} }
TY - JOUR ID - 858028 AU - Vojtylová, Terézia - Dospivová, Dana - Třísková, Olga - Pilařová, Iveta - Lubal, Přemysl - Farková, Marta - Trnková, Libuše - Táborský, Petr PY - 2009 TI - Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine JF - Chem. Papers VL - 63 IS - 6 SP - 731-737 EP - 731-737 SN - 03666352 KW - oligonucleotides KW - adenine KW - cytosine KW - protonation KW - thermodynamics KW - chemometrics UR - http://www.springerlink.com/content/b163418v38432172/?p=afb907946c874228a3c45a57420400e5&pi=15 N2 - Acidobasic properties of purine and pyrimidine bases (adenine, cytosine) and relevant nucleosides (adenosine, cytidine) were studied by means of glass-electrode potentiometry and the respective dissociation constants were determined under given experimental conditions (I = 0.1 M (NaCl), t = (25.0 +/- 0.1) degrees of Celsius): adenine (pK_HL = 9.65 +/- 0.04, pK_H2L = 4.18 +/- 0.04), adenosine (pK_H2L = 3.59 +/- 0.05), cytosine (pK_H2L = 4.56 +/- 0.01), cytidine (pK_H2L = 4.16 +/- 0.02). In addition, thermodynamic parameters for bases were calculated. Acidobasic behavior of oligonucleotides ((CAC)3, A3C6, C3A3C3) was studied under the same experimental conditions by molecular absorption spectroscopy. pH-dependent spectral datasets were analyzed by means of advanced chemometric techniques (EFA, MCR-ALS) and the presence of hemiprotonated species concerning (C-C+) a non-canonical pair (i-motif) in titled oligonucleotides was proposed in order to explain experimental data obtained according to literature. ER -
VOJTYLOVÁ, Terézia, Dana DOSPIVOVÁ, Olga TŘÍSKOVÁ, Iveta PILAŘOVÁ, Přemysl LUBAL, Marta FARKOVÁ, Libuše TRNKOVÁ and Petr TÁBORSKÝ. Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine. \textit{Chem. Papers}. 2009, vol.~63, No~6, p.~731-737. ISSN~0366-6352.
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