Revealing the mysteries of the RS20L lectin from Ralstonia
solanacearum ;	a molecular modelling study

D 2009

Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study

ĎURECH, Michal, Jan ADAM, Zdeněk KŘÍŽ, Nikola KOSTLÁNOVÁ, Jaroslav KOČA et. al.

Základní údaje

Originální název

Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study

Název česky

Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study

Autoři

ĎURECH, Michal, Jan ADAM, Zdeněk KŘÍŽ, Nikola KOSTLÁNOVÁ, Jaroslav KOČA a Michaela WIMMEROVÁ

Vydání

Brno, XIII. Setkání biochemiků a molekulárních biologů, s. 109-109, 2009

Nakladatel

Masarykova Univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-4830-0

Klíčová slova česky

lectin engineering; molecular modeling; thermodynamics

Klíčová slova anglicky

lectin engineering; molecular modeling; thermodynamics

Změněno: 9. 4. 2010 09:24, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

ORIG CZ

V originále

Our research is focused on bacterial lectin RS20L from Ralstonia solanacearum.The RS20L lectin displays a different structure from these two, and no structure homologue was found for this lectin in the bacterial lectin realm. Its fold resembles animal realm galectins. The native lectin structure was crystallized, and during the experimental preparation of a recombinant form and attempts of functional characterization, several cases of very peculiar behavior were recorded extremely high stability of trimeric form, irreversible loss of binding properties after purification, etc. The research focuses on investigating the general and binding properties of the RS20L lectin that could help to explain this unusual behaviour. The study was performed by means of computational chemistry and molecular modelling. Several promising hints were found that could, upon further investigation, help to decrypt the reasons behind the lectin behavior.

Česky

Prace se zabyva lektinem RS20L z bakterie Ralstonia solanacearum. Tento lektin se v rade pripadu chova velmi neobvykle. Metody molekuloveho modelovani aplikovane na tento system napomohly objevit nekolik dalsich informaci, ktere by mohly vysvetlovat vazebne i obecne chovani tohoto proteinu.

Návaznosti

GA303/09/1168, projekt VaV

Název: Lektiny z lidských patogenů - struktura, funkce, inženýrství

Investor: Grantová agentura ČR, Lektidy z lidských patogenů - struktura, funkce, inženýrství

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

ĎURECH, Michal, Jan ADAM, Zdeněk KŘÍŽ, Nikola KOSTLÁNOVÁ, Jaroslav KOČA a Michaela WIMMEROVÁ. Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study. In XIII. Setkání biochemiků a molekulárních biologů. Brno: Masarykova Univerzita, 2009, s. 109. ISBN 978-80-210-4830-0.

@inproceedings{858664,
   author = {Ďurech, Michal and Adam, Jan and Kříž, Zdeněk and Kostlánová, Nikola and Koča, Jaroslav and Wimmerová, Michaela},
   address = {Brno},
   booktitle = {XIII. Setkání biochemiků a molekulárních biologů},
   keywords = {lectin engineering; molecular modeling; thermodynamics},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-4830-0},
   pages = {109-109},
   publisher = {Masarykova Univerzita},
   title = {Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study},
   year = {2009}
}

TY  - JOUR
ID  - 858664
AU  - Ďurech, Michal - Adam, Jan - Kříž, Zdeněk - Kostlánová, Nikola - Koča, Jaroslav - Wimmerová, Michaela
PY  - 2009
TI  - Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study
PB  - Masarykova Univerzita
CY  - Brno
SN  - 9788021048300
KW  - lectin engineering
KW  - molecular modeling
KW  - thermodynamics
N2  - Our research is focused on bacterial lectin RS20L from Ralstonia solanacearum.The RS20L lectin displays a different structure from these two, and no structure homologue was found for this lectin in the bacterial lectin realm. Its fold resembles animal realm galectins. The native lectin structure was crystallized, and during the experimental preparation of a recombinant form and attempts of functional characterization, several cases of very peculiar behavior were recorded extremely high stability of trimeric form, irreversible loss of binding properties after purification, etc. The research focuses on investigating the general and binding properties of the RS20L lectin that could help to explain this unusual behaviour. The study was performed by means of computational chemistry and molecular modelling. Several promising hints were found that could, upon further investigation, help to decrypt the reasons behind the lectin behavior.
ER  -

ĎURECH, Michal, Jan ADAM, Zdeněk KŘÍŽ, Nikola KOSTLÁNOVÁ, Jaroslav KOČA a Michaela WIMMEROVÁ. Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study. In \textit{XIII. Setkání biochemiků a molekulárních biologů}. Brno: Masarykova Univerzita, 2009, s.~109. ISBN~978-80-210-4830-0.

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