SUV39h-independent association of HP1b with
fibrillarin-positive nucleolar regions

J 2010

SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions

HARNIČAROVÁ, Andrea; Eva BÁRTOVÁ; Gabriela GALIOVÁ; Radka UHLÍŘOVÁ; Pavel MATULA et. al.

Basic information

Original name

SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions

Authors

HARNIČAROVÁ, Andrea (703 Slovakia); Eva BÁRTOVÁ (203 Czech Republic); Gabriela GALIOVÁ (203 Czech Republic); Radka UHLÍŘOVÁ (203 Czech Republic); Pavel MATULA (203 Czech Republic, guarantor, belonging to the institution) and Stanislav KOZUBEK (203 Czech Republic)

Edition

Chromosoma, Springer, 2010, 0009-5915

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 4.196

RIV identification code

RIV/00216224:14330/10:00051235

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1007/s00412-009-0252-2

UT WoS

000277786400001

Keywords in English

SUV39h; heterochromatin protein 1; nucleoli; cell nucleus

Abstract

In the original language

Heterochromatin protein 1 (HP1), which binds to sites of histone H3 lysine 9 (H3K9) methylation, is primarily responsible for gene silencing and the formation of heterochromatin. We observed that HP1beta is located in both the chromocenters and fibrillarin-positive nucleoli interiors. However, HP1alfa and HP1gamma occupied fibrillarin-positive compartments to a lesser extent, corresponding to the distinct levels of HP1 subtypes at the promoter of rDNA genes. Deficiency of histone methyltransferases SUV39h, and/or inhibition of histone deacetylases (HDACi) decreased HP1beta and H3K9 trimethylation at chromocenters, but not in fibrillarin-positive regions that co-localized with RNA Pol I. Similarly, SUV39h- and HDACi-dependent nucleolar rearrangement and inhibition of rDNA transcription did not affect the association between HP1beta - and fibrillarin. The presence of HP1beta in nucleoli is likely connected with transcription of ribosomal genes and with the role of fibrillarin in nucleolar processes.

Links

LC535, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

Investor: Ministry of Education, Youth and Sports of the CR, Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii

2B06052, research and development project

Name: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)

Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images

Cite

HARNIČAROVÁ, Andrea; Eva BÁRTOVÁ; Gabriela GALIOVÁ; Radka UHLÍŘOVÁ; Pavel MATULA and Stanislav KOZUBEK. SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions. Chromosoma. Springer, 2010, vol. 119, No 3, p. 227-241. ISSN 0009-5915. Available from: https://dx.doi.org/10.1007/s00412-009-0252-2.

@article{858785,
   author = {Harničarová, Andrea and Bártová, Eva and Galiová, Gabriela and Uhlířová, Radka and Matula, Pavel and Kozubek, Stanislav},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s00412-009-0252-2},
   keywords = {SUV39h; heterochromatin protein 1; nucleoli; cell nucleus},
   language = {eng},
   issn = {0009-5915},
   journal = {Chromosoma},
   title = {SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions},
   url = {http://www.springerlink.com/content/03353h53h300j70k/},
   volume = {119},
   year = {2010}
}

TY  - JOUR
ID  - 858785
AU  - Harničarová, Andrea - Bártová, Eva - Galiová, Gabriela - Uhlířová, Radka - Matula, Pavel - Kozubek, Stanislav
PY  - 2010
TI  - SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions
JF  - Chromosoma
VL  - 119
IS  - 3
SP  - 227-241
EP  - 227-241
PB  - Springer
SN  - 00095915
KW  - SUV39h
KW  - heterochromatin protein 1
KW  - nucleoli
KW  - cell nucleus
UR  - http://www.springerlink.com/content/03353h53h300j70k/
L2  - http://www.springerlink.com/content/03353h53h300j70k/
N2  - Heterochromatin protein 1 (HP1), which binds to sites of histone H3 lysine 9 (H3K9) methylation, is primarily responsible for gene silencing and the formation of heterochromatin. We observed that HP1beta is located in both the chromocenters and fibrillarin-positive nucleoli interiors. However, HP1alfa and HP1gamma occupied fibrillarin-positive compartments to a lesser extent, corresponding to the distinct levels of HP1 subtypes at the promoter of rDNA genes. Deficiency of histone methyltransferases SUV39h, and/or inhibition of histone deacetylases (HDACi) decreased HP1beta and H3K9 trimethylation at chromocenters, but not in fibrillarin-positive regions that co-localized with RNA Pol I. Similarly, SUV39h- and HDACi-dependent nucleolar rearrangement and inhibition of rDNA transcription did not affect the association between HP1beta - and fibrillarin. The presence of HP1beta in nucleoli is likely connected with transcription of ribosomal genes and with the role of fibrillarin in nucleolar processes.
ER  -

HARNIČAROVÁ, Andrea; Eva BÁRTOVÁ; Gabriela GALIOVÁ; Radka UHLÍŘOVÁ; Pavel MATULA and Stanislav KOZUBEK. SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions. \textit{Chromosoma}. Springer, 2010, vol.~119, No~3, p.~227-241. ISSN~0009-5915. Available from: https://dx.doi.org/10.1007/s00412-009-0252-2.

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