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@proceedings{862218,
author = {Bouchal, Pavel and Struhárová, Iva and Budinská, Eva and Šedo, Ondrej and Vyhlídalová, Tereza and Zdráhal, Zbyněk and van Spanning, Rob and Kučera, Igor},
booktitle = {2009 SPS Scientific Meeting: "Proteome Dynamics: Protein Quantification in Time and Space"},
keywords = {proteome Paracoccus denitrificans},
language = {cze},
title = {Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches},
url = {http://www.swissproteomicsociety.org/congress09/},
year = {2009}
}
TY - CONF
ID - 862218
AU - Bouchal, Pavel - Struhárová, Iva - Budinská, Eva - Šedo, Ondrej - Vyhlídalová, Tereza - Zdráhal, Zbyněk - van Spanning, Rob - Kučera, Igor
PY - 2009
TI - Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
KW - proteome Paracoccus denitrificans
UR - http://www.swissproteomicsociety.org/congress09/
N2 - The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-, NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
ER -
BOUCHAL, Pavel, Iva STRUHÁROVÁ, Eva BUDINSKÁ, Ondrej ŠEDO, Tereza VYHLÍDALOVÁ, Zbyněk ZDRÁHAL, Rob VAN SPANNING a Igor KUČERA. Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches. In \textit{2009 SPS Scientific Meeting: ''Proteome Dynamics: Protein Quantification in Time and Space''}. 2009.
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