Srs2: the Odd-Job Man in DNA repair

MARINI PALOMEQUE, María Victoria a Lumír KREJČÍ. Srs2: the Odd-Job Man in DNA repair. DNA Repair. ELSEVIER, 2010, roč. 9, č. 3, 3 s. ISSN 1568-7864.

Základní údaje

• Originální název: Srs2: the Odd-Job Man in DNA repair
• Název česky: Srs2: Muž pro všechno v DNA opravě
• Autoři: MARINI PALOMEQUE, María Victoria (858 Uruguay, domácí) a Lumír KREJČÍ (203 Česká republika, garant, domácí).
• Vydání: DNA Repair, ELSEVIER, 2010, 1568-7864.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Nizozemské království
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 4.293
• Kód RIV: RIV/00216224:14310/10:00043104
• Organizační jednotka: Přírodovědecká fakulta
• UT WoS: 000276016800008
• Klíčová slova anglicky: DNA repair; DNA damage; replication; genomic instability
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

The genome is constantly threatened by various damaging agents and maintaining its integrity is crucial in all organisms. Several repair pathways have been implicated in the removal of different types of lesions from DNA. Among them, homologous recombination (HR) plays a key role in repair of double-strand breaks (DSBs). Although HR is a highly important repair mechanism, it has to be regulated to prevent it from interfering with other DNA repair pathways, generating toxic intermediates, or blocking the progression of the replication fork. Therefore, it is not surprising that cells have evolved mechanisms that counteract untimely HR events. In the yeast Saccharomyces cerevisiae, one of the pathways responsible for regulation of HR requires the action of the SRS2 gene product. Mutations in the SRS2 gene exhibit pleiotropic recombination phenotypes ranging from anti-recombinogenic in one aspect to pro-recombinogenic in another. In addition to its role in HR, Srs2 is also involved in other DNA metabolism processes, including post-replication repair (PRR), preservation of replication fork integrity, DNA-damage checkpoint responses, DNA triplet maintenance and non-homologous end joining (NHEJ). The aim of this review is to summarize the current knowledge about Srs2 and its effect on multiple DNA metabolic processes that generally affect genome stability. It would appear that Srs2 functions as an Odd-Job Man in these processes to make sure that the jobs proceed when and where they are needed.

Anotace česky

The genome is constantly threatened by various damaging agents and maintaining its integrity is crucial in all organisms. Several repair pathways have been implicated in the removal of different types of lesions from DNA. Among them, homologous recombination (HR) plays a key role in repair of double-strand breaks (DSBs). Although HR is a highly important repair mechanism, it has to be regulated to prevent it from interfering with other DNA repair pathways, generating toxic intermediates, or blocking the progression of the replication fork. Therefore, it is not surprising that cells have evolved mechanisms that counteract untimely HR events. In the yeast Saccharomyces cerevisiae, one of the pathways responsible for regulation of HR requires the action of the SRS2 gene product. Mutations in the SRS2 gene exhibit pleiotropic recombination phenotypes ranging from anti-recombinogenic in one aspect to pro-recombinogenic in another. In addition to its role in HR, Srs2 is also involved in other DNA metabolism processes, including post-replication repair (PRR), preservation of replication fork integrity, DNA-damage checkpoint responses, DNA triplet maintenance and non-homologous end joining (NHEJ). The aim of this review is to summarize the current knowledge about Srs2 and its effect on multiple DNA metabolic processes that generally affect genome stability. It would appear that Srs2 functions as an Odd-Job Man in these processes to make sure that the jobs proceed when and where they are needed.

Návaznosti

• GA301/09/1917, projekt VaV: Název: Štěpení replikačních-rekombinačních DNA meziproduktů a jejich úloha při nestabilitě genomu

Investor: Grantová agentura ČR, Štěpení replikačních-rekombinačních DNA meziproduktů a jejich úloha při nestabilitě genomu

• GD203/09/H046, projekt VaV: Název: Biochemie na rozcestí mezi in silico a in vitro

Investor: Grantová agentura ČR, Biochemie na rozcestí mezi in silico a in vitro

• LC06030, projekt VaV: Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

• ME10048, projekt VaV: Název: Vliv post-translačních modifikací na DNA opravu a rekombinaci.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vliv post-translačních modifikací na DNA opravu a rekombinaci., Program výzkumu a vývoje KONTAKT (ME)

• MSM0021622413, záměr: Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím