| KRYUKOV, Fedor, Darina OČADLÍKOVÁ, Lucie KOVÁŘOVÁ, Ivana BUREŠOVÁ, Eva MATĚJKOVÁ, Roman HÁJEK a Jaroslav MICHÁLEK. In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 Pulsed Dendritic Cells. The Open Cancer Immunology Journal. Bentham Science Publishers Ltd., 2009, roč. 2, č. 1, s. 31-36. ISSN 1876-4010. |
Další formáty:
BibTeX
LaTeX
RIS
@article{866179,
author = {Kryukov, Fedor and Očadlíková, Darina and Kovářová, Lucie and Burešová, Ivana and Matějková, Eva and Hájek, Roman and Michálek, Jaroslav},
article_number = {1},
keywords = {multiple myeloma; dendritic cell; Muc1; immunotherapy},
language = {eng},
issn = {1876-4010},
journal = {The Open Cancer Immunology Journal},
title = {In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 Pulsed Dendritic Cells},
volume = {2},
year = {2009}
}
TY - JOUR
ID - 866179
AU - Kryukov, Fedor - Očadlíková, Darina - Kovářová, Lucie - Burešová, Ivana - Matějková, Eva - Hájek, Roman - Michálek, Jaroslav
PY - 2009
TI - In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 Pulsed Dendritic Cells
JF - The Open Cancer Immunology Journal
VL - 2
IS - 1
SP - 31-36
EP - 31-36
PB - Bentham Science Publishers Ltd.
SN - 18764010
KW - multiple myeloma
KW - dendritic cell
KW - Muc1
KW - immunotherapy
N2 - We studied antigen-specific and HLA-A2-restricted cytotoxic activity against MUC1-positive ARH77 myeloma cell line in vitro. A HLA-A2 specific MUC1-derived nonapetide (139TSAPDTRPA147) was used as a tumor-associated antigen. Myeloma-specific cytotoxic activity of MUC1-reactive CTL was established by repeated stimulation of CTL via dendritic cells loaded with MUC1-derived nonapeptide. We were able to demonstrate that MUC1-reactive T cells can be identified and expanded using a relatively simple in vitro techniques. Antigen-specific stimulation via dendritic cells, immunomagnetic sorting and rapid expansion of CTL without loosing of their specific immune activity can be used clinically even in poorly immunogenic diseases such as MM.
ER -
KRYUKOV, Fedor, Darina OČADLÍKOVÁ, Lucie KOVÁŘOVÁ, Ivana BUREŠOVÁ, Eva MATĚJKOVÁ, Roman HÁJEK a Jaroslav MICHÁLEK. In Vitro Activation of Cytotoxic T Lymphocytes By Muc1 Pulsed Dendritic Cells. \textit{The Open Cancer Immunology Journal}. Bentham Science Publishers Ltd., 2009, roč.~2, č.~1, s.~31-36. ISSN~1876-4010.
|
