MASAŘÍK, Michal, Dalibor HÚSKA, Vojtěch ADAM, V. Jaroslav BURDA, Tomáš ECKSCHLAGER, Marie STIBOROVÁ and René KIZEK. Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions. International Journal of Molecular Medicine. 2009, vol. 24, Suppl. 1, p. S49, 1 pp. ISSN 1107-3756. |
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@article{868047, author = {Masařík, Michal and Húska, Dalibor and Adam, Vojtěch and Burda, V. Jaroslav and Eckschlager, Tomáš and Stiborová, Marie and Kizek, René}, article_number = {Suppl. 1}, keywords = {Doxorubicin; DNA; electrochemistry; drug-DNA interaction}, language = {eng}, issn = {1107-3756}, journal = {International Journal of Molecular Medicine}, title = {Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions}, volume = {24}, year = {2009} }
TY - JOUR ID - 868047 AU - Masařík, Michal - Húska, Dalibor - Adam, Vojtěch - Burda, V. Jaroslav - Eckschlager, Tomáš - Stiborová, Marie - Kizek, René PY - 2009 TI - Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions JF - International Journal of Molecular Medicine VL - 24 IS - Suppl. 1 SP - S49 EP - S49 SN - 11073756 KW - Doxorubicin KW - DNA KW - electrochemistry KW - drug-DNA interaction N2 - Anthracyclines including doxorubicin are commonly used in the treatment of a number of diverse malignant. There is compelling evidence that cellular DNA is the primary target for anthracyclines. In this study we aimed at investigation of intercalation of doxorubicin into DNA in vitro by using of square wave voltammetry coupled with adsorptive transfer stripping technique. Based on the decline of the signal of adenine and cytosine, we indicated binding of doxorubicin into dsDNA. The electrochemical method was further utilized for detection of doxorubicin-DNA adducts in neuroblastoma cells. Prior to electrochemical analysis dsDNA was carefully isolated from these cells to avoid modification of DNA bases. We observed significant (a=0.05) difference in DNA signals after intercalation of doxorubicin between resistant and sensitive cells. For more comprehensive understanding of the intercalation, computational experiments using design for testability approach with correction on dispersion interaction were performed. Several molecular structures were explored and clear preference for very stable stacked structures was observed. ER -
MASAŘÍK, Michal, Dalibor HÚSKA, Vojtěch ADAM, V. Jaroslav BURDA, Tomáš ECKSCHLAGER, Marie STIBOROVÁ and René KIZEK. Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions. \textit{International Journal of Molecular Medicine}. 2009, vol.~24, Suppl. 1, p.~S49, 1 pp. ISSN~1107-3756.
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