2010
Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells
BERNARD, Vladan, Jiřina ŠKORPÍKOVÁ, Vojtěch MORNSTEIN a Iva SLANINOVÁZákladní údaje
Originální název
Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells
Autoři
BERNARD, Vladan (203 Česká republika, garant, domácí), Jiřina ŠKORPÍKOVÁ (203 Česká republika, domácí), Vojtěch MORNSTEIN (203 Česká republika, domácí) a Iva SLANINOVÁ (203 Česká republika, domácí)
Vydání
Ultrasonics, 2010, 0041-624X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10610 Biophysics
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.599
Kód RIV
RIV/00216224:14110/10:00043266
Organizační jednotka
Lékařská fakulta
UT WoS
000273954700004
Klíčová slova anglicky
Carcinoma cells; Cisplatin; Ultrasound; Viability; MTT test
Změněno: 12. 1. 2011 12:31, doc. Mgr. Vladan Bernard, Ph.D.
Anotace
V originále
Abstract- The effects of low-power ultrasound, the anti-cancer drug cisplatin, and their combined application were studied in two lines of human ovarian carcinoma cells, A2780 and A2780cis. Four modes of treatment were used: exposure to ultrasonic field, application of cisplatin, exposure to ultrasound followed by cisplatin, and presence of cisplatin followed by exposure to application ultrasound. Ultrasound was used at intensities of 0.5 W/cm(2) and 1.0 W/cm(2) for 10 min, cisplatin was applied at concentrations of 1 mu M and 6 mu M per cell suspension treated in A2780 and cisplatin-resistant A2780cis cells, respectively. The results of each experimental treatment were assessed by the resultant cell viability related to the viability of control cells, using a standard MTT test. It was shown that a combined effect of ultrasound and cisplatin was more effective than that of ultrasound or cisplatin alone. It also appeared that the order of application played a role, with the cisplatin-ultrasound treatment lowering cell viability more than the ultrasound-cisplatin treatment. It can be assumed that the exposure of cells to a low-power ultrasonic field has an immediate effect on the structure of cell surfaces and, consequently, on entry of cisplatin into the cell.
Návaznosti
| GA305/05/2030, projekt VaV |
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| LC06077, projekt VaV |
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| MUNI/C/0092/2009, interní kód MU |
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