| KOZUBENKO, Nataliya, Karolína TURNOVCOVÁ, Miroslava KAPCALOVA, Olena BUTENKO, Miroslava ANDĚROVÁ, Vendula RUSNÁKOVÁ, Mikael KUBISTA, Aleš HAMPL, Pavla JENDELOVÁ a Eva SYKOVÁ. Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors. Cell Transplantation. 2010, roč. 19, č. 4, s. 471-486. ISSN 0963-6897. |
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@article{869300,
author = {Kozubenko, Nataliya and Turnovcová, Karolína and Kapcalova, Miroslava and Butenko, Olena and Anděrová, Miroslava and Rusnáková, Vendula and Kubista, Mikael and Hampl, Aleš and Jendelová, Pavla and Syková, Eva},
article_number = {4},
keywords = {human embryonic stem cells; neural differentiation; fluorescent activated cell sorting; RT-PCR; transplantation},
language = {eng},
issn = {0963-6897},
journal = {Cell Transplantation},
title = {Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors},
volume = {19},
year = {2010}
}
TY - JOUR
ID - 869300
AU - Kozubenko, Nataliya - Turnovcová, Karolína - Kapcalova, Miroslava - Butenko, Olena - Anděrová, Miroslava - Rusnáková, Vendula - Kubista, Mikael - Hampl, Aleš - Jendelová, Pavla - Syková, Eva
PY - 2010
TI - Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors
JF - Cell Transplantation
VL - 19
IS - 4
SP - 471-486
EP - 471-486
SN - 09636897
KW - human embryonic stem cells
KW - neural differentiation
KW - fluorescent activated cell sorting
KW - RT-PCR
KW - transplantation
N2 - Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating neural precursors from the CCTL14 line of human embryonic stem cells (hESCs). These neural precursors could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and lineage specification. The analyzed cells were used for transplantation into the injured rodent brain; the tumorigenicity of the transplanted cells was apparently eliminated following long-term culture. These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies..
ER -
KOZUBENKO, Nataliya, Karolína TURNOVCOVÁ, Miroslava KAPCALOVA, Olena BUTENKO, Miroslava ANDĚROVÁ, Vendula RUSNÁKOVÁ, Mikael KUBISTA, Aleš HAMPL, Pavla JENDELOVÁ a Eva SYKOVÁ. Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors. \textit{Cell Transplantation}. 2010, roč.~19, č.~4, s.~471-486. ISSN~0963-6897.
|
