Bioaccumulation test of persistent organic pollutants in
Eisenia fetida: chemical analysis issues

a 2009

Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues

VLČKOVÁ, Klára a Jakub HOFMAN

Základní údaje

Originální název

Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues

Název česky

Bioakumulace perzistentních organických polutantů v žížale Eisenia fetida: otázky chemické analýzy

Autoři

VLČKOVÁ, Klára a Jakub HOFMAN

Vydání

SETAC Europe 19th Annual Meeting, 31 May - 4 June 2009, Goteborg, Sweden. In : Abstract Book p.164-165, 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10511 Environmental sciences

Stát vydavatele

Švédsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

bioakumulace; Eisenia fetida; perzistentní organické polutanty; kontaminace půdy

Klíčová slova anglicky

bioacumulation; Eisenia fetida; persistent organic pollutants; soil spiking

Příznaky

Mezinárodní význam

Změněno: 2. 9. 2010 10:11, prof. RNDr. Jakub Hofman, Ph.D.

Anotace

V originále

Bioaccumulation of pollutants in earthworms must be seriously considered in ecological risk assessment, because it may result to toxic effects for earthworms and to biomagnification of food chains. Nowadays, OECD bioaccumulation test guideline is finishing. However, we think that there are still some issues which are worth to consider when measuring the bioaccumulation of POPs in earthworms. At this poster, these issues are discussed using the results gathered (lets say by the way) throughout our bioaccumulation experiments with spiked soils, Eisenia fetida and GC/MS analysis. Firstly, it is necessary to control the soil spiking: (1) analyze spiking solution, (2) check real soil concentrations after spiking and contamination homogeneity, and (3) adjust moisture loss during spiking. We suggest here a procedure how to do these. Using described approach the variability of POPs distribution in the experimental soil was kept below 10%. When analyzing POPs in exposed Eisena fetida it is necessary to know (1) variability and (2) background concentrations in worms. We performed 10 independent replicates of the bioaccumulation test and we found RSD 21%, 26%, 19%, 12%, and 30% for the accumulated concentrations of phenanthrene, pyrene, PCB 153, lindane, and DDT respectively. This is relatively high and it suggests that more than 3 replicates recommended in the new OECD guideline should be used definitely. In worms from the control, we found some POPs which never exceeded 800 ng/gdw. And finally, all necessary controls of recovery must run in analysis of both worms and soil. Cca 1/3 total loss of analytes during worms extraction and clean-up was mainly (70-90%) caused by nitrogen blow-down steps. It is also recommended to check the matrix effect on the analytical response.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

VLČKOVÁ, Klára a Jakub HOFMAN. Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues. In SETAC Europe 19th Annual Meeting, 31 May - 4 June 2009, Goteborg, Sweden. In : Abstract Book p.164-165. 2009.

@proceedings{873459,
   author = {Vlčková, Klára and Hofman, Jakub},
   booktitle = {SETAC Europe 19th Annual Meeting, 31 May - 4 June 2009, Goteborg, Sweden. In : Abstract Book p.164-165},
   keywords = {bioacumulation; Eisenia fetida; persistent organic pollutants; soil spiking},
   language = {eng},
   title = {Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues},
   year = {2009}
}

TY  - CONF
ID  - 873459
AU  - Vlčková, Klára - Hofman, Jakub
PY  - 2009
TI  - Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues
KW  - bioacumulation
KW  - Eisenia fetida
KW  - persistent organic pollutants
KW  - soil spiking
N2  - Bioaccumulation of pollutants in earthworms must be seriously considered in ecological risk assessment, because it may result to toxic effects for earthworms and to biomagnification of food chains. Nowadays, OECD bioaccumulation test guideline is finishing. However, we think that there are still some issues which are worth to consider when measuring the bioaccumulation of POPs in earthworms. At this poster, these issues are discussed using the results gathered (lets say by the way) throughout our bioaccumulation experiments with spiked soils, Eisenia fetida and GC/MS analysis. Firstly, it is necessary to control the soil spiking: (1) analyze spiking solution, (2) check real soil concentrations after spiking and contamination homogeneity, and (3) adjust moisture loss during spiking. We suggest here a procedure how to do these. Using described approach the variability of POPs distribution in the experimental soil was kept below 10%. When analyzing POPs in exposed Eisena fetida it is necessary to know (1) variability and (2) background concentrations in worms. We performed 10 independent replicates of the bioaccumulation test and we found RSD 21%, 26%, 19%, 12%, and 30% for the accumulated concentrations of phenanthrene, pyrene, PCB 153, lindane, and DDT respectively. This is relatively high and it suggests that more than 3 replicates recommended in the new OECD guideline should be used definitely. In worms from the control, we found some POPs which never exceeded 800 ng/gdw. And finally, all necessary controls of recovery must run in analysis of both worms and soil. Cca 1/3 total loss of analytes during worms extraction and clean-up was mainly (70-90%) caused by nitrogen blow-down steps. It is also recommended to check the matrix effect on the analytical response.
ER  -

VLČKOVÁ, Klára a Jakub HOFMAN. Bioaccumulation test of persistent organic pollutants in Eisenia fetida: chemical analysis issues. In \textit{SETAC Europe 19th Annual Meeting, 31 May - 4 June 2009, Goteborg, Sweden. In : Abstract Book p.164-165}. 2009.

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