| HORKÁ, Marie, Filip RŮŽIČKA, Veronika HOLÁ, Vladislav KAHLE, Dana MORAVCOVÁ a Karel ŠLAIS. Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2009, roč. 81, č. 16, s. 6897–6904. ISSN 0003-2700. |
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@article{875884,
author = {Horká, Marie and Růžička, Filip and Holá, Veronika and Kahle, Vladislav and Moravcová, Dana and Šlais, Karel},
article_location = {Washington, D.C., USA},
article_number = {16},
keywords = {Capillary Electromigration Techniques; Proteins; Microorganisms; Dynamic Modification; Chromophoric Non-ionogenic Surfactant},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant},
volume = {81},
year = {2009}
}
TY - JOUR
ID - 875884
AU - Horká, Marie - Růžička, Filip - Holá, Veronika - Kahle, Vladislav - Moravcová, Dana - Šlais, Karel
PY - 2009
TI - Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant
JF - Analytical Chemistry
VL - 81
IS - 16
SP - 6897–6904
EP - 6897–6904
PB - American Chemical Society
SN - 00032700
KW - Capillary Electromigration Techniques
KW - Proteins
KW - Microorganisms
KW - Dynamic Modification
KW - Chromophoric Non-ionogenic Surfactant
N2 - Chromophoric non-ionogenic surfactant poly(ethylene glycol) 3-(2-hydroxy-5-n-octylphenylazo)-benzoate, HOPAB, has been prepared and used as a buffer additive for a dynamic modification of proteins and/or microorganisms including Escherichia coli, Staphylococcus epidermidis (biofilm positive and negative), the strains of yeast cells, Candida albicans, Candida parapsilosis (biofilm positive and negative) during a capillary electrophoresis and a capillary isoelectric focusing with UV detection at 326 nm. Minimum detectable amount has been assessed lower than picograms of proteins and lower than a hundred cells injected into a separation capillary. The introduced labeling method facilitates CIEF separation of microorganisms from the clinical sample of the infected urine at their clinically important level in the pH gradient pH range 2-5 and their subsequent cultivation.
ER -
HORKÁ, Marie, Filip RŮŽIČKA, Veronika HOLÁ, Vladislav KAHLE, Dana MORAVCOVÁ a Karel ŠLAIS. Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2009, roč.~81, č.~16, s.~6897–6904. ISSN~0003-2700.
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