Staphylococcus microti sp. nov., isolated from the common vole
(Microtus arvalis)

J 2010

Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)

NOVÁKOVÁ, Dana; Roman PANTŮČEK; Zdeněk HUBÁLEK; Enevold FALSEN; Hans-Juergen BUSSE et. al.

Basic information

Original name

Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)

Name in Czech

Staphylococcus microti sp. nov., izlovaný z hraboše Microtus arvalis

Authors

NOVÁKOVÁ, Dana (203 Czech Republic, belonging to the institution); Roman PANTŮČEK (203 Czech Republic, belonging to the institution); Zdeněk HUBÁLEK (203 Czech Republic); Enevold FALSEN (752 Sweden); Hans-Juergen BUSSE (40 Austria); Peter SCHUMANN (276 Germany) and Ivo SEDLÁČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Systematic and Evolutionary Microbiology, Reading, United Kingdom, Society for General Microbiology, 2010, 1466-5026

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 1.930

RIV identification code

RIV/00216224:14310/10:00043517

Organization unit

Faculty of Science

UT WoS

000276328700016

Keywords in English

Staphylococcus microti; molecular taxonomy

Abstract

In the original language

Two strains of Gram-positive cocci were isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus Staphylococcus and assigned Staphylococcus muscae as the nearest relative. A detailed characterization done by ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)5 primer differentiated the two strains from all described staphylococci. DNA-DNA hybridization with the type strain of S. muscae demonstrated that the two strains should be considered as members of a novel species (26.8% reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C15 : 0, iso-C17 : 0, anteiso- C15 : 0, C18 : 2v6,9c/anteiso-C18 : 0, C18 : 0 and C18 : 1v9c. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is Staphylococcus microti sp. nov.; the type strain is 4005-LJ(m)T (5CCM 4903T 5CCUG 55861T 5DSM 22147T).

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

Cite

NOVÁKOVÁ, Dana; Roman PANTŮČEK; Zdeněk HUBÁLEK; Enevold FALSEN; Hans-Juergen BUSSE; Peter SCHUMANN and Ivo SEDLÁČEK. Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis). International Journal of Systematic and Evolutionary Microbiology. Reading, United Kingdom: Society for General Microbiology, 2010, vol. 60, No 3, p. 566-573. ISSN 1466-5026.

@article{876077,
   author = {Nováková, Dana and Pantůček, Roman and Hubálek, Zdeněk and Falsen, Enevold and Busse, HansandJuergen and Schumann, Peter and Sedláček, Ivo},
   article_location = {Reading, United Kingdom},
   article_number = {3},
   keywords = {Staphylococcus microti; molecular taxonomy},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)},
   url = {http://ijs.sgmjournals.org/cgi/content/abstract/60/3/566},
   volume = {60},
   year = {2010}
}

TY  - JOUR
ID  - 876077
AU  - Nováková, Dana - Pantůček, Roman - Hubálek, Zdeněk - Falsen, Enevold - Busse, Hans-Juergen - Schumann, Peter - Sedláček, Ivo
PY  - 2010
TI  - Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 60
IS  - 3
SP  - 566-573
EP  - 566-573
PB  - Society for General Microbiology
SN  - 14665026
KW  - Staphylococcus microti
KW  - molecular taxonomy
UR  - http://ijs.sgmjournals.org/cgi/content/abstract/60/3/566
N2  - Two strains of Gram-positive cocci were isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus Staphylococcus and assigned Staphylococcus muscae as the nearest relative. A detailed characterization done by ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)5 primer differentiated the two strains from all described staphylococci. DNA-DNA hybridization with the type strain of S. muscae demonstrated that the two strains should be considered as members of a novel species (26.8% reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C15 : 0, iso-C17 : 0, anteiso- C15 : 0, C18 : 2v6,9c/anteiso-C18 : 0, C18 : 0 and C18 : 1v9c. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is Staphylococcus microti sp. nov.; the type strain is 4005-LJ(m)T (5CCM 4903T 5CCUG 55861T 5DSM 22147T).
ER  -

NOVÁKOVÁ, Dana; Roman PANTŮČEK; Zdeněk HUBÁLEK; Enevold FALSEN; Hans-Juergen BUSSE; Peter SCHUMANN and Ivo SEDLÁČEK. Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis). \textit{International Journal of Systematic and Evolutionary Microbiology}. Reading, United Kingdom: Society for General Microbiology, 2010, vol.~60, No~3, p.~566-573. ISSN~1466-5026.

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