Simultaneous HPLC determination of tolbutamide, phenacetin and their metabolites as markers of cytochromes 1A2 and 2C6/11 in rat liver perfusate

JUŘICA, Jan, Jiří KONEČNÝ, Lucia ZAHRADNÍKOVÁ and Josef TOMANDL. Simultaneous HPLC determination of tolbutamide, phenacetin and their metabolites as markers of cytochromes 1A2 and 2C6/11 in rat liver perfusate. Journal of Pharmaceutical and Biomedical Analysis. 2010, vol. 52, No 4, p. 557-564. ISSN 0731-7085.

Basic information

• Original name: Simultaneous HPLC determination of tolbutamide, phenacetin and their metabolites as markers of cytochromes 1A2 and 2C6/11 in rat liver perfusate
• Name in Czech: Současné HPLC stanovení tolbutamidu, fenacetinu a jejich metabolitů jako markerů metabolické aktivity cytochromu P450 1A2 a 2C6 v perfuzátu potkaních jater
• Authors: JUŘICA, Jan (203 Czech Republic, guarantor, belonging to the institution), Jiří KONEČNÝ (203 Czech Republic), Lucia ZAHRADNÍKOVÁ (703 Slovakia) and Josef TOMANDL (203 Czech Republic, belonging to the institution).
• Edition: Journal of Pharmaceutical and Biomedical Analysis, 2010, 0731-7085.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.733
• RIV identification code: RIV/00216224:14110/10:00043518
• Organization unit: Faculty of Medicine
• UT WoS: 000276057300017
• Keywords (in Czech): CYP2C6; CYP1A2; metabolická aktivita; marker
• Keywords in English: CYP2C6; CYP1A2; metabolic activity; marker
• Tags: isolated perfused rat liver, metabolic activity, phenacetin, tolbutamide
• Tags: International impact, Reviewed

Abstract

The new original method for assessment the metabolic activity of both rat CYP1A2 and CYP2C6/11 is presented in the paper. The method of simultaneous determination of phenacetin, tolbutamide and their metabolites is rapid simple, repeatable and precious and sensitive enough for pharmacokinetic studies.

Abstract (in Czech)

Publikovaným výsledkem je nová metodika současného stanovení markerů metabolické aktivity forem CYP1A2 a CYP2C6/11 u potkana. Jedná se o stanovení fenacetinu a paracetamolu (marker potkaní i lidské CYP1A2) a tolbutamidu a hydroxytolbutamidu (marker potkaní CYP2C6/11, lidské CYP2C9). Metoda je rychlá, jednoduchá, opakovatelná a dostatečně přesná pro farmakokinetické studie.

Links

• GAP206/10/0057, research and development project: Name: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze

Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.