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@proceedings{877314,
author = {Dobisová, Tereza and Hejátko, Jan},
booktitle = {7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us"},
keywords = {CKI1; EMS mutagenesis; forward genetics},
language = {eng},
isbn = {978-80-7375-310-8},
note = {oral presentation},
title = {IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH},
url = {http://phd.czweb.org/phd09/},
year = {2009}
}
TY - CONF
ID - 877314
AU - Dobisová, Tereza - Hejátko, Jan
PY - 2009
TI - IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH
SN - 9788073753108
N1 - oral presentation
KW - CKI1
KW - EMS mutagenesis
KW - forward genetics
UR - http://phd.czweb.org/phd09/
N2 - Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.
ER -
DOBISOVÁ, Tereza a Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In \textit{7th International Conference of Ph.D Students on Experimental Plant Biology ''News - what the plants told us''}. 2009. ISBN~978-80-7375-310-8.
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