IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1
EXPRESION USING FORWARD GENETIC APPROACH

a 2009

IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

DOBISOVÁ, Tereza and Jan HEJÁTKO

Basic information

Original name

IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

Authors

DOBISOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us" 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/09:00043563

Organization unit

Faculty of Science

ISBN

978-80-7375-310-8

Keywords (in Czech)

CKI1; EMS mutageneze; přímá genetika

Keywords in English

CKI1; EMS mutagenesis; forward genetics

Změněno: 2/5/2011 09:44, Mgr. Tereza Dobisová, Ph.D.

Abstract

V originále

Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.

Links

GD204/08/H054, research and development project

Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

LC06034, research and development project

Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

DOBISOVÁ, Tereza and Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In 7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us". 2009. ISBN 978-80-7375-310-8.

@proceedings{877314,
   author = {Dobisová, Tereza and Hejátko, Jan},
   booktitle = {7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us"},
   keywords = {CKI1; EMS mutagenesis; forward genetics},
   language = {eng},
   isbn = {978-80-7375-310-8},
   note = {oral presentation},
   title = {IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH},
   url = {http://phd.czweb.org/phd09/},
   year = {2009}
}

TY  - CONF
ID  - 877314
AU  - Dobisová, Tereza - Hejátko, Jan
PY  - 2009
TI  - IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH
SN  - 9788073753108
N1  - oral presentation
KW  - CKI1
KW  - EMS mutagenesis
KW  - forward genetics
UR  - http://phd.czweb.org/phd09/
N2  - Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.
ER  -

DOBISOVÁ, Tereza and Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In \textit{7th International Conference of Ph.D Students on Experimental Plant Biology ''News - what the plants told us''}. 2009. ISBN~978-80-7375-310-8.

Detailed Information on Publication Record