IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1
EXPRESION USING FORWARD GENETIC APPROACH

a 2009

IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

DOBISOVÁ, Tereza a Jan HEJÁTKO

Základní údaje

Originální název

IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

Autoři

DOBISOVÁ, Tereza (203 Česká republika, domácí) a Jan HEJÁTKO (203 Česká republika, garant, domácí)

Vydání

7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us" 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/09:00043563

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7375-310-8

Klíčová slova česky

CKI1; EMS mutageneze; přímá genetika

Klíčová slova anglicky

CKI1; EMS mutagenesis; forward genetics

Změněno: 2. 5. 2011 09:44, Mgr. Tereza Dobisová, Ph.D.

Anotace

V originále

Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.

Návaznosti

GD204/08/H054, projekt VaV

Název: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Grantová agentura ČR, Molekulární mechanismy proliferace a diferenciace buněk

LC06034, projekt VaV

Název: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Regulace morfogeneze rostlinných buněk a orgánů

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

DOBISOVÁ, Tereza a Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In 7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us". 2009. ISBN 978-80-7375-310-8.

@proceedings{877314,
   author = {Dobisová, Tereza and Hejátko, Jan},
   booktitle = {7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us"},
   keywords = {CKI1; EMS mutagenesis; forward genetics},
   language = {eng},
   isbn = {978-80-7375-310-8},
   note = {oral presentation},
   title = {IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH},
   url = {http://phd.czweb.org/phd09/},
   year = {2009}
}

TY  - CONF
ID  - 877314
AU  - Dobisová, Tereza - Hejátko, Jan
PY  - 2009
TI  - IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH
SN  - 9788073753108
N1  - oral presentation
KW  - CKI1
KW  - EMS mutagenesis
KW  - forward genetics
UR  - http://phd.czweb.org/phd09/
N2  - Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.
ER  -

DOBISOVÁ, Tereza a Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In \textit{7th International Conference of Ph.D Students on Experimental Plant Biology ''News - what the plants told us''}. 2009. ISBN~978-80-7375-310-8.

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