a 2010

Preparation of microsamples for elemental analysis of biological samples

FOLTYNOVÁ, Pavla, Ondřej PEŠ, Tomáš VACULOVIČ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA et. al.

Basic information

Original name

Preparation of microsamples for elemental analysis of biological samples

Name in Czech

Příprava mikrovzorků pro elementální analýzu biologických materiálů

Authors

FOLTYNOVÁ, Pavla (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

25th International Symposium on MicroScale Bioseparations MSB 2010, 2010

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00043578

Organization unit

Faculty of Science

ISBN

978-80-254-6631-5

Keywords in English

off-line coupling; ICP MS; SALD; mass spectrometry; proteins; biological samples
Změněno: 9/4/2013 10:14, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

New approaches of sample introduction to inductively-coupled plasma mass spectrometry which are especially suitable for minute amount of samples are presented. Detailed characterization of SALD ICP MS has shown large signal stability over a wide range of laser power density values, the independence of signal on acquisition modes and linear dynamic range over six orders of magnitude. SALD ICP MS with low limits of detection (0.1-14 fmol for Cr, Cu, Co, Fe, Ni, Sn and Zn) is suitable for off-line coupling with capillary electrophoresis (CE) for speciation analysis or selective detection of metals. Applicability to biological samples analysis was demonstrated on investigation of the role of copper ions to regulate cytotoxicity of disulfiram in myeloid leukemia cells. SALD ICP MS allows rapid, sensitive and reproducible method for quantitative analysis of metals with very low consumption of sample. The advantages of SALD are also sample archiving and possibility of consequent analysis in different detection modes, such as MALDI MS or fluorescence).

Links

GA203/09/1025, research and development project
Name: Multidetekční platforma pro mikrokolonové separace proteinů a peptidů
Investor: Czech Science Foundation, Multidetection platform for microcolumn separation of proteins and peptides
MSM0021622411, plan (intention)
Name: Studium a aplikace plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek
Investor: Ministry of Education, Youth and Sports of the CR, Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations