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@proceedings{878426,
author = {Klumpler, Tomáš and Pekárová, Blanka and Marek, Jaromír and Borkovcová, Pavla and Janda, Lubomír and Hejátko, Jan},
booktitle = {Kolokvium - Struktura 2009},
keywords = {histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure},
language = {eng},
title = {Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana},
year = {2009}
}
TY - CONF
ID - 878426
AU - Klumpler, Tomáš - Pekárová, Blanka - Marek, Jaromír - Borkovcová, Pavla - Janda, Lubomír - Hejátko, Jan
PY - 2009
TI - Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
KW - histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure
N2 - The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices.
ER -
KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Pavla BORKOVCOVÁ, Lubomír JANDA a Jan HEJÁTKO. Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana. In \textit{Kolokvium - Struktura 2009}. 2009. ISSN~1211-5894.
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