Effect of quaternary benzo[c]phenanthridine alkaloids
sanguilutine and chelilutine on normal and cancer cells

J 2010

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ, Iva SLANINOVÁ et. al.

Základní údaje

Originální název

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

Název česky

Vliv kvarterních benzo [c] fenanthridinových alkaloidů sanguilutinu a chelilutinu na normální a nádorové buňky

Autoři

SLUNSKÁ, Zdenka (203 Česká republika), Eva GELNAROVÁ (203 Česká republika), Jindřiška HAMMEROVÁ (203 Česká republika, domácí), Eva TÁBORSKÁ (203 Česká republika, domácí) a Iva SLANINOVÁ (203 Česká republika, garant, domácí)

Vydání

Toxicology in Vitro, Amsterodam, Elsevier Ltd. 2010, 0887-2333

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.546

Kód RIV

RIV/00216224:14110/10:00043675

Organizační jednotka

Lékařská fakulta

UT WoS

000276668500001

Klíčová slova anglicky

benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant

Změněno: 14. 1. 2011 09:51, prof. MUDr. Iva Slaninová, Ph.D.

Anotace

V originále

Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid. All tested alkaloids, at concentrations of 0.1 and 1 ug/ml, did not increase ROS production. However, pre-incubation with all alkaloids, at all tested concentrations, with the exception of sanguilutine and chelerythrine at 1 ug/ml, decreased oxidative stress caused by H2O2. These findings indicate high anti-proliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction; to the contrary, both alkaloids showed anti-oxidative effects.

Návaznosti

GA525/08/0819, projekt VaV

Název: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA

Investor: Grantová agentura ČR, Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA

LC06077, projekt VaV

Název: Centrum chemické genetiky

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum chemické genetiky

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ a Iva SLANINOVÁ. Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells. Toxicology in Vitro. Amsterodam: Elsevier Ltd., 2010, roč. 24, č. 3, s. 697-706. ISSN 0887-2333.

@article{878739,
   author = {Slunská, Zdenka and Gelnarová, Eva and Hammerová, Jindřiška and Táborská, Eva and Slaninová, Iva},
   article_location = {Amsterodam},
   article_number = {3},
   keywords = {benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant},
   language = {eng},
   issn = {0887-2333},
   journal = {Toxicology in Vitro},
   title = {Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4},
   volume = {24},
   year = {2010}
}

TY  - JOUR
ID  - 878739
AU  - Slunská, Zdenka - Gelnarová, Eva - Hammerová, Jindřiška - Táborská, Eva - Slaninová, Iva
PY  - 2010
TI  - Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells
JF  - Toxicology in Vitro
VL  - 24
IS  - 3
SP  - 697-706
EP  - 697-706
PB  - Elsevier Ltd.
SN  - 08872333
KW  - benzo(c)phenanthridine alkaloids
KW  - sanguilutine
KW  - chelilutine
KW  - cytotoxicity
KW  - oxidative stress
KW  - antioxidant
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4
N2  - Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid. All tested alkaloids, at concentrations of 0.1 and 1 ug/ml, did not increase ROS production. However, pre-incubation with all alkaloids, at all tested concentrations, with the exception of sanguilutine and chelerythrine at 1 ug/ml, decreased oxidative stress caused by H2O2. These findings indicate high anti-proliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction; to the contrary, both alkaloids showed anti-oxidative effects.
ER  -

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ a Iva SLANINOVÁ. Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells. \textit{Toxicology in Vitro}. Amsterodam: Elsevier Ltd., 2010, roč.~24, č.~3, s.~697-706. ISSN~0887-2333.

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