| KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA, Lyudmila KANCHEVA, Ivo ŘÍHA a Luboslav STARKA. Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances. Journal of Steroid Biochemistry and Molecular Biology. England: Elsevier Science, 2010, roč. 119, 1-2, s. 35-44. ISSN 0960-0760. Dostupné z: https://dx.doi.org/10.1016/j.jsbmb.2009.12.006. |
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@article{880129,
author = {Kancheva, Radmila and Hill, Martin and Novák, Zdeněk and Chrastina, Jan and Velikova, Marta and Kancheva, Lyudmila and Říha, Ivo and Starka, Luboslav},
article_location = {England},
article_number = {1-2},
doi = {http://dx.doi.org/10.1016/j.jsbmb.2009.12.006},
keywords = {Steroids; Cerebrospinal fluid (CSF); Plasma; Metabolome; GC-MS},
language = {eng},
issn = {0960-0760},
journal = {Journal of Steroid Biochemistry and Molecular Biology},
title = {Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances},
volume = {119},
year = {2010}
}
TY - JOUR
ID - 880129
AU - Kancheva, Radmila - Hill, Martin - Novák, Zdeněk - Chrastina, Jan - Velikova, Marta - Kancheva, Lyudmila - Říha, Ivo - Starka, Luboslav
PY - 2010
TI - Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances
JF - Journal of Steroid Biochemistry and Molecular Biology
VL - 119
IS - 1-2
SP - 35-44
EP - 35-44
PB - Elsevier Science
SN - 09600760
KW - Steroids
KW - Cerebrospinal fluid (CSF)
KW - Plasma
KW - Metabolome
KW - GC-MS
N2 - To compare the predictivity of the neuroactive steroids in the cerebrospinal fluid and peripheral blood for the diagnostics of CNS disturbances, eighteen unconjugated steroids were quantified in the cerebrospinal fluid (CSF) from the 3rd ventricle and 18 unconjugated steroids and 7 steroid polar conjugates were measured in the serum using GC-MS and RIA. Eight postmenopausal women (56-78 years of age) and 7 men (22-88 years of age) with hydrocephalus were enrolled in the study. The sensitivity of the method ranged from low femtogram to low picogram levels depending on the steroid fragmentation pattern. Using multivariate regression, a model for simultaneous prediction of the CSF steroids from the serum steroids was completed. Then, the penetrability of the individual steroids across the blood-brain-barrier was evaluated and the sources of various brain steroids were estimated. Our data show that a part of the steroids may be synthesized de novo in the CNS. However, substantial part of the steroid metabolites may be synthesized in the CNS from the steroid precursors or directly transported from the periphery. The CNS in situ synthesis and transport from periphery might be complementary in some cases, i.e. brain synthesis might provide minimum level of steroids, which are indispensable for the CNS functions.
ER -
KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA, Lyudmila KANCHEVA, Ivo ŘÍHA a Luboslav STARKA. Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances. \textit{Journal of Steroid Biochemistry and Molecular Biology}. England: Elsevier Science, 2010, roč.~119, 1-2, s.~35-44. ISSN~0960-0760. Dostupné z: https://dx.doi.org/10.1016/j.jsbmb.2009.12.006.
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