Comparative evaluation of automated ribotyping and RAPD-PCR for
typing of Lactobacillus spp. occurring in dental caries

J 2010

Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries

ŠVEC, Pavel, Martina KUKLETOVÁ a Ivo SEDLÁČEK

Základní údaje

Originální název

Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries

Autoři

ŠVEC, Pavel (203 Česká republika, garant, domácí), Martina KUKLETOVÁ (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Springer, 2010, 0003-6072

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.673

Kód RIV

RIV/00216224:14310/10:00043797

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000276902800009

Klíčová slova anglicky

Dental caries; Lactobacillus; Randomly amplified polymorphic DNA (RAPD-PCR); RiboPrinter; Ribotyping

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2011 07:30, doc. RNDr. Pavel Švec, Ph.D.

Anotace

V originále

A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (Švec et al., Folia Microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the RiboPrinter microbial characterization system and by randomly amplified polymorphic DNA fingerprinting (RAPD-PCR) with M13 primer to evaluate these techniques for characterization of lactobacilli associated with dental caries. Ribotyping revealed 55 riboprints among the analysed group. The automatic identification process performed by the RiboPrinter system identified 18 strains to the species level, however cluster analysis divided obtained ribotype patterns into individual clusters mostly corresponding to the species assignment of particular strains. RAPD-PCR fingerprints revealed by the individual Lactobacillus spp. showed higher variability than the ribotype patterns and the fingerprint profiles generated by the analysed species were distributed among one to four clusters. In conclusion, ribotyping is shown to be more convenient for the identification purposes while RAPD-PCR fingerprinting results indicate this method is a better tool for typing of Lactobacillus spp. strains occurring in dental caries.

Návaznosti

GA310/09/0657, projekt VaV

Název: Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici

Investor: Grantová agentura ČR, Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

1M0528, projekt VaV

Název: Stomatologické výzkumné centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Stomatologické výzkumné centrum

Citovat

ŠVEC, Pavel, Martina KUKLETOVÁ a Ivo SEDLÁČEK. Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Springer, 2010, roč. 98, č. 1, s. 85-92. ISSN 0003-6072.

@article{881114,
   author = {Švec, Pavel and Kukletová, Martina and Sedláček, Ivo},
   article_number = {1},
   keywords = {Dental caries; Lactobacillus; Randomly amplified polymorphic DNA (RAPD-PCR); RiboPrinter; Ribotyping},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
   title = {Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries},
   url = {http://www.springerlink.com/content/lv85174706rg56p1/?p=31e5ef171e0e42678745e028ba8d3e31&pi=8},
   volume = {98},
   year = {2010}
}

TY  - JOUR
ID  - 881114
AU  - Švec, Pavel - Kukletová, Martina - Sedláček, Ivo
PY  - 2010
TI  - Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries
JF  - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL  - 98
IS  - 1
SP  - 85-92
EP  - 85-92
PB  - Springer
SN  - 00036072
KW  - Dental caries
KW  - Lactobacillus
KW  - Randomly amplified polymorphic DNA (RAPD-PCR)
KW  - RiboPrinter
KW  - Ribotyping
UR  - http://www.springerlink.com/content/lv85174706rg56p1/?p=31e5ef171e0e42678745e028ba8d3e31&pi=8
N2  - A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (Švec et al., Folia Microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the RiboPrinter microbial characterization system and by randomly amplified polymorphic DNA fingerprinting (RAPD-PCR) with M13 primer to evaluate these techniques for characterization of lactobacilli associated with dental caries. Ribotyping revealed 55 riboprints among the analysed group. The automatic identification process performed by the RiboPrinter system identified 18 strains to the species level, however cluster analysis divided obtained ribotype patterns into individual clusters mostly corresponding to the species assignment of particular strains. RAPD-PCR fingerprints revealed by the individual Lactobacillus spp. showed higher variability than the ribotype patterns and the fingerprint profiles generated by the analysed species were distributed among one to four clusters. In conclusion, ribotyping is shown to be more convenient for the identification purposes while RAPD-PCR fingerprinting results indicate this method is a better tool for typing of Lactobacillus spp. strains occurring in dental caries.
ER  -

ŠVEC, Pavel, Martina KUKLETOVÁ a Ivo SEDLÁČEK. Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Springer, 2010, roč.~98, č.~1, s.~85-92. ISSN~0003-6072.

Podrobný výpis o publikaci