| KOTAŠKOVÁ, Jana, Boris TICHÝ, Martin TRBUŠEK, Hana FRANCOVÁ, Jitka KABÁTHOVÁ, Jitka MALČÍKOVÁ, Michael DOUBEK, Yvona BRYCHTOVÁ, Jiří MAYER a Šárka POSPÍŠILOVÁ. High Expression of Lymphocyte-Activation Gene 3 in Chronic Lymphocytic Leukemia Cells is Associated with Unmutated IGHV and Reduced Treatment-Free Survival. The journal of molecular diagnostics. Bethesda, MD: American Society for Investigative Pathology/Association for Molecular Pathology, 2010, roč. 12, č. 3, s. 328-334. ISSN 1525-1578. |
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@article{883000,
author = {Kotašková, Jana and Tichý, Boris and Trbušek, Martin and Francová, Hana and Kabáthová, Jitka and Malčíková, Jitka and Doubek, Michael and Brychtová, Yvona and Mayer, Jiří and Pospíšilová, Šárka},
article_location = {Bethesda, MD},
article_number = {3},
keywords = {IGVH; CLL; LAG3},
language = {eng},
issn = {1525-1578},
journal = {The journal of molecular diagnostics.},
title = {High Expression of Lymphocyte-Activation Gene 3 in Chronic Lymphocytic Leukemia Cells is Associated with Unmutated IGHV and Reduced Treatment-Free Survival.},
volume = {12},
year = {2010}
}
TY - JOUR
ID - 883000
AU - Kotašková, Jana - Tichý, Boris - Trbušek, Martin - Francová, Hana - Kabáthová, Jitka - Malčíková, Jitka - Doubek, Michael - Brychtová, Yvona - Mayer, Jiří - Pospíšilová, Šárka
PY - 2010
TI - High Expression of Lymphocyte-Activation Gene 3 in Chronic Lymphocytic Leukemia Cells is Associated with Unmutated IGHV and Reduced Treatment-Free Survival.
JF - The journal of molecular diagnostics.
VL - 12
IS - 3
SP - 328-334
EP - 328-334
PB - American Society for Investigative Pathology/Association for Molecular Pathology
SN - 15251578
KW - IGVH
KW - CLL
KW - LAG3
N2 - Chronic lymhocytic leukemia (CLL) is characterized by a monoclonal expansion of mature B-lymphocytes. Mutational status of the immunoglobulin variable heavy chain region (IGVH) gene stratifies CLL patients into two prognostic groups. We performed microarray analysis of CLL cells using the Agilent platform to detect the most important gene expression differences regarding IGVH status in CLL cells. We analyzed a cohort of 118 CLL patients with different IGVH mutational status and completely characterized all described prognostic markers using expression microarrays and quantitative real-time RT-PCR (reverse transcription PCR). We detected lymphocyte-activation gene 3 (LAG3) as a novel prognostic marker: LAG3 high expression in CLL cells correlates with unmutated IGVH (P<0.0001) and reduced treatment free survival (P=0.0087). Furthermore, quantitative real-time RT-PCR analysis identified a gene-set (LAG3, LPL, ZAP70) whose overexpression is assigned to unmutated IGVH with 90% specificity (P<0.0001).
ER -
KOTAŠKOVÁ, Jana, Boris TICHÝ, Martin TRBUŠEK, Hana FRANCOVÁ, Jitka KABÁTHOVÁ, Jitka MALČÍKOVÁ, Michael DOUBEK, Yvona BRYCHTOVÁ, Jiří MAYER a Šárka POSPÍŠILOVÁ. High Expression of Lymphocyte-Activation Gene 3 in Chronic Lymphocytic Leukemia Cells is Associated with Unmutated IGHV and Reduced Treatment-Free Survival. \textit{The journal of molecular diagnostics.}. Bethesda, MD: American Society for Investigative Pathology/Association for Molecular Pathology, 2010, roč.~12, č.~3, s.~328-334. ISSN~1525-1578.
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