SLOVÁČKOVÁ, Jana, Diana GROCHOVÁ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA and Jana ŠMARDOVÁ. Transactivation by temperature dependent p53 mutants in yeast and human cells. In 21st Meeting of the European Association for Cancer Research, Oslo, Norway, Eur. J. Cancer. 2010. ISSN 1359-6349.
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Basic information
Original name Transactivation by temperature dependent p53 mutants in yeast and human cells
Authors SLOVÁČKOVÁ, Jana (203 Czech Republic, belonging to the institution), Diana GROCHOVÁ (203 Czech Republic), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Jana ŠMARDOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition 21st Meeting of the European Association for Cancer Research, Oslo, Norway, Eur. J. Cancer, 2010.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30200 3.2 Clinical medicine
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 9.386
RIV identification code RIV/00216224:14310/10:00044309
Organization unit Faculty of Science
ISSN 1359-6349
UT WoS 000279148400026
Keywords (in Czech) p53; teplotně závislé mutace; FASAY
Keywords in English p53; temperature-dependent mutations; FASAY
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Jan Šmarda, CSc., učo 1223. Changed: 24/1/2011 07:21.
Abstract
In response to stress signals, p53 controls essential cell functions by regulating expression of its target genes. Full or partial loss of the p53 function in cancer cells usually results from mutations of the p53 gene. Some of them are temperature-dependent, allowing reactivation of the p53 function in certain temperature.We analyzed transactivation of p21-, bax- and mdm2 genes by 23 temperature-dependent p53 mutants in transiently transfected human lung H1299 cells (p53-null) by luciferase reporter assay.We confirmed temperature-dependency and discriminative character of the most p53 mutants and stratified them into four functional groups. Despite the differences of yeast and human cells, they allowed similar transactivation rates to the p53 mutants, thus providing evidence that functional analysis of separated alleles in yeast is valuable tool for assessment of the human p53 status.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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