D 2010

Spectroscopic study of protonation of cytosine-containing oligonucleotides

HOLUBOVÁ, Sylvie, Přemysl LUBAL, Marta FARKOVÁ, Libuše TRNKOVÁ, Blanka HÉGROVÁ et. al.

Basic information

Original name

Spectroscopic study of protonation of cytosine-containing oligonucleotides

Authors

HOLUBOVÁ, Sylvie (203 Czech Republic), Přemysl LUBAL (203 Czech Republic, guarantor), Marta FARKOVÁ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic), Blanka HÉGROVÁ (203 Czech Republic) and Petra KRISTOVÁ (203 Czech Republic)

Edition

Pardubice, Sborníi 14. česko - slovenské spektroskopické konference, 100 pp. 2010

Publisher

Spektroskopická společnost Jana Marka Marci

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00044422

Organization unit

Faculty of Science

ISBN

978-80-7395-282-2

Keywords in English

spectroscopy; cytosine; protonation constant
Změněno: 2/8/2010 16:14, RNDr. Kamila Neplechová, Ph.D.

Abstract

V originále

Cytosine is an important building block of nucleic acids taking part in the process of genetic information transfer and transfer of energy of biologically important compounds by means of coenzymes. Due to acidobasic properties of bioligands, the presence of protonated species is dependent on the protonation constants. It was also shown that oligonucleotides (ODNs) concerning cytosine can form the so called structural i motif as a consequence of probable stabilization of the secondary structure of biomacromolecules. The aim of this work was to determine the protonation constants of ODNs varying the number of bases in order to see their influence on thermodynamic stability of studied ligands.

Links

LC06035, research and development project
Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.
Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)