Spectroscopic study of protonation of cytosine-containing oligonucleotides

HOLUBOVÁ, Sylvie, Přemysl LUBAL, Marta FARKOVÁ, Libuše TRNKOVÁ, Blanka HÉGROVÁ and Petra KRISTOVÁ. Spectroscopic study of protonation of cytosine-containing oligonucleotides. In Sborníi 14. česko - slovenské spektroskopické konference. Pardubice: Spektroskopická společnost Jana Marka Marci, 2010, 100 pp. ISBN 978-80-7395-282-2.

Basic information

• Original name: Spectroscopic study of protonation of cytosine-containing oligonucleotides
• Authors: HOLUBOVÁ, Sylvie (203 Czech Republic), Přemysl LUBAL (203 Czech Republic, guarantor), Marta FARKOVÁ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic), Blanka HÉGROVÁ (203 Czech Republic) and Petra KRISTOVÁ (203 Czech Republic).
• Edition: Pardubice, Sborníi 14. česko - slovenské spektroskopické konference, 100 pp. 2010.
• Publisher: Spektroskopická společnost Jana Marka Marci

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00044422
• Organization unit: Faculty of Science
• ISBN: 978-80-7395-282-2
• Keywords in English: spectroscopy; cytosine; protonation constant

Abstract

Cytosine is an important building block of nucleic acids taking part in the process of genetic information transfer and transfer of energy of biologically important compounds by means of coenzymes. Due to acidobasic properties of bioligands, the presence of protonated species is dependent on the protonation constants. It was also shown that oligonucleotides (ODNs) concerning cytosine can form the so called structural i motif as a consequence of probable stabilization of the secondary structure of biomacromolecules. The aim of this work was to determine the protonation constants of ODNs varying the number of bases in order to see their influence on thermodynamic stability of studied ligands.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)